1987 Fiscal Year Final Research Report Summary
Effects of Liposomal SOD on human polymorphonuclear neutrophils and monocytes
| Project/Area Number |
60570443
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
YANAI MASANORI Nagasaki University School of Medicine, 医学部, 講師 (90128231)
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| Project Period (FY) |
1985 – 1987
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| Keywords | Liposomal SOD / Polymorphonuclear neutrophil / Monocyte / Kawasaki disease / Targeting therapy / Radical oxgens / Chemiluminescence response / 貧食殺菌能 |
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| Research Abstract |
We examined in vitro the effects of liposomal bovine copper superoxide dismutase(SOD) on human polymorphonuclear neutrophil(PMN) and monocyte functions. Liposomal encapsulated SOD (50<micrn>g/ml) suspension soppressed the peak of luminol-amplified chemiluminescence(CL) response produced by PMNs during phagocytosis of opsonized zymosan by 52.6%. The supernatant of liposomal SOD suspension inhibited the CL response and superoxide anion production more than its sediment. Liposomal SOD suspension might therefore have contained much smaller liposomes than pellet which is composed of big liposomes.
The peak of CL response in PMNs which were preincubated with liposomal SOD(50<micrn>g/ml) for 30 minutes at 37゜C was suppressed by 73.4%. However, there was no significant interference with PMN and monocyte functions, when PMNs and monocytes were preincubated with liposomal SOD at the concentration of 5 <micrn>g per ml.
Two patients having Kawasaki disease were treated with liposomal SOD. This therapy might be effective for fever and other symptoms and doses not have toxicity.
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