1987 Fiscal Year Final Research Report Summary
Ultrastructural and histological studies on degeneration and repair of the articular cartilage
| Project/Area Number |
60570700
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
KANIE Ryoichi Nagoya City Univ. Medical School, Assoc. Prof., 医学部, 助教授 (80080000)
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| Co-Investigator(Kenkyū-buntansha) |
INAMOTO Masahiro Nagoya City Univ. Medical School, Researcher, 医学部, 研究員
KAWAI Hiroshi Nagoya City Univ. Medical School, Researcher, 医学部, 研究員
ITOH Takashi Nagoya City Univ. Medical School, Assistant, 医学部, 助手 (90151513)
TANEDA Yoichi Nagoya City Univ. Medical School, Assistant, 医学部, 助手 (40145735)
IMAIZUMI Tsukasa Nagoya City Univ. Medical School, Lecturer, 医学部, 講師 (70080033)
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| Project Period (FY) |
1985 – 1987
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| Keywords | Degeneration of Articular Cartilage / Repair of Articular Cartilage / Aging of Articular Cartilage / Process of Degeneration / Electron Microscopic Findings of Articular Cartilage / Degenerative Substances in Chondrocyte / 実験的関節血腫 / 二足肥満ラット / 軟骨細胞変性過程 |
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| Research Abstract |
To understand the pathogenesis of various jount diseases, it is important to get the characteristic findings and make clear the mechanism of aging and degeneration of the articular cartilage. I have studied this problem using the transmission electron microscope (TEM) and the scanning electron microscope (SEM) on specimens from experimental animals and humans.
The results are: 1) Under TEM observation, the normal young rat's cartilagenous cell is abundant in well developed rough surfaced endoplasmic reticulum(ER). The terminal parts of ER are cystically swollen and contain an amorphous substance. This suggests vigorous protein synthesis and cell activity. 2) Some chondrocytes of middle-aged rats show pre-hydropic degeneration with abundance of hyaline substances. 3) In the deeper zone of the adult human cartilage, characteristic findings are prominent accumulation of glycogen granules, an abundance of fine filamentous substances in a bundle, and a few lipid droplets. These findings may
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mean an accumulation of surplus carbohydrates,proteins and fatty substances, respectively, and seem to indicate an abnormal metabolism or the process of degeneration. 4) The chondrocytes of old rats tend to contain nubecular substances, occupying large parts of the cytoplasm, and considered to be degenerative substances. These cells are seen to suffer various degrees of degeneration and gradually lose their shape. In some such cells, a lot of filamentous substances are scattered among the nubecular substances. In the matrix around such cells, closely outside the lacunae, a lot of fine dot-like substances can be seen. With higher magnification, some such particles are seen to be debris of disintegrated chondrocytes. 5) In osteoarthritic cartilage, those degenerative findings are distinct. 6) Under SEM observation, with age, the cartilagenous surface of normal rats gradually becomes rough with decreased matrix substance and bare collagen fibrils. The surface of osteoarthritic cartilage shows various degrees of non-uniform roughening, and seems different from that of old-aged people, in which the roughening is almost uniform. Less
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