Cytochemical Study on Muller Cells in the Retinal Degeneration and Atrophy

1986 Fiscal Year Final Research Report Summary

• Project/Area Number: 60570832
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kyoto University
• Principal Investigator: UENO SATOKI Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (20109010)
• Co-Investigator(Kenkyū-buntansha): HIRATA AKIRA Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (10181158) ; ISHIGOUOKA HITOSHI Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (80135590)
• Project Period (FY): 1985 – 1986
• Keywords: Muller Cells / Cytochemistry / Experimental Retinal Degeneration / Diathermy / Laser Photocoagulation / High Voltage Electron Microscope

Research Abstract

We have found that potassium-independent paranitrophenylphosphatase and glucose 6 phosphatase activities were restricted to the endoplasmic reticulum of retinal glial Muller cells in the guinea pig retina. Utilizing these newly developed cytochemical method, it could be easier to identify Muller cells after experimental retinal diathermy or laser photocoagulation. Muller cells have revealed mainly three different pathological processes corresponding to strength of stimulation. These pathological changes were also observed under high voltage electron microscopy for obtaining three dimensional view. We have also examine the participation of Muller cells in the transitional areas of the retina such as optic nerve-retinal or cilio-retinal region. In both areas, it could be easily revealed that Muller cells play major role in the connection between adjustcent epithelial components. Consequently, this cytochemical method might be useful for examination of Muller cells not only pathologically but also embryologically, cytologically.

Research Products

• [Publications] 平田昭,石郷岡均,北岡隆,仁平美果,上野聰樹: 日本眼科紀要. 10号. (1987)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishigouoka,H;Hirata,A.;Kitaoka,T.;Ueno,S.: Documenta Ophthalmologica. (1986)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ueno,S.;Ishigouoka,H.;Hirata,A.;Kitaoka,T.;Nihira,M.;Ueck,M.: The Proceedings of Symposium on Neuroendocrine System.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishigouoka,H.,Hrata,A.,Kitaoka,T.,Nihira,M.,Ueno,S.: "Cytochemical study of the Muller cells during repairing process after retinal coagulation." Documenta Ophthalmologica.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hirata,A.,Ishigouoka,H.,Kitaoka,T.,Nihira,M.,Ueno,S.: "Ultracytochemical studies of the ora serrata region in the guinea pig eye." Folia Ophthalmologica Japan. 10. (1987)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ueno,S.,Ishigouoka,H.,Hirata,A.,Kitaoka,T.,Nihira,M.,Ueck,M.: "Histo- and cytochemical study on retinal glial Muller cells of the guinea pig in the normal and pathological conditions" Proceedings of Symposium On Neuroendocrine System 1986.
  • Description: 「研究成果報告書概要(欧文)」より