1986 Fiscal Year Final Research Report Summary

Hormonal Influence on actin-binding protein expression in muscle and non-muscle cells

Research Project

Project/Area Number	60580123
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	物質生物化学
Research Institution	Akita University, School of medicine
Principal Investigator	KOBAYASHI Ryoji Department of Biochemistry,Akita University School of Medicine, 医学部, 助教授 (00020917)
Project Period (FY)	1985 – 1986
Keywords	Alpha-actinin / Filamin / Vinculin / Gelsolin / Calpactin / Myogenesis / Thyroid hormone / 分化
Research Abstract	The isoform of skeletal muscle alpha-actinin present during chick embryogenesis were analyzed. Chicken embryonic muscle at ages 8-15 days contain an embryo-specfic isoform of alpha- actinin. Developmental sequence of alpha-actinin isoforms expression was also studied. In extracts of skeletal muscle from 8-10 days embryo only the embryo-specific isoform was detected. In extracts from 15 days embryo, it was noted that both the embryonic form and adult form co-existed. However, in 21 days embryo, the embryonic form disappeared, and the adult form was detected. These data suggested that the embryonic and the adult form of alpha-actinin are distinct gene products, and that during skeletal myogenesis in ovo, one class of alpha-actinin is replaced by a new class of alpha-actinin polypeptide,and the latter is maintained into adulthood. Filamin, gelsolin, vinculin and calpacin expression during skeletal myogenesis was also studied.

(2 results)