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1988 Fiscal Year Final Research Report Summary

Development of a Specific Micro-determination Method of Gizzerosine, an Inducer of Gizzard Erosion in Chicken

Research Project

Project/Area Number 60860009
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionThe University of Tokyo

Principal Investigator

NAITO Hiroshi  Professor of Nutritional Chemistry, Faculty of Agriculture, The University of Tokyo, 農学部, 教授 (80034445)

Project Period (FY) 1985 – 1986
Keywordsgizzerosine / histamine / high-performance liquid chromatography / gizzard erosion / histamine receptor / fish meal
Research Abstract

1. A fluorometric determination method of gizzerosine was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 g of gizzerosine in Fish meals was quantitated with the recovery of 95-102 %. fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine.
2. Employing the newly developed determination method, the conditions of gizzerosine formation in fish meals were examined. When the pH of fish meat was slightly acidic and when the temperature during drying was more than 100゜C, significant amount of gizzerosine was formed in the fish meal.
3. If lysine or ascorbic acid was included in fish meat before drying, the formation of gizzerosine was prevented. This method made it possible to produce low-gizzerosine fish meal.
4. Mucosal cells of chicken proventriculus were prepared employing collagenase digestion method. Gizzerosione enhanced the oxygen uptake by these cells and the activity was inhibited by antagonists of histamine H_2-receptor. This suggests that gizzard erosion induced by gizzerosine can be prevented by cimetidine and its related compounds. This is anothor possible method to prevent gizzard erosion in chicks.
5. The above-mentioned determination method was relatively complex. So, another method was tried to be developed. By using ion-exchange high performance liquid chromatography, gizzerosine was determined within 1 or 2 days. A practical procedure for wide usage of this method is now being developed.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Yoshinori Ito,;Tadashi Noguchi,;Hiroshi Naito: Analttical Biochemistry. 151. 28-31 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Mori,;Takeshi Sugai,;Yukari Maeda,;Tomomi Okazaki,;Tadashi Noguchi,;Hiroshi Naito: Tetrahedron. 41. 5307-5311 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuneo Hino,;Tadashi Noguchi,;Hiroshi Naito: Poultry Science. 66. 548-551 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Ito,;Tadashi Noguchi Hiroshi Naito: Agricultural Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Naito,;Yoshinori Ito,;Tadashi Noguchi: "Amino-Carbonyl Reaction in Food and Biological Systems---Proceedings of the 3rd International Symposium on the Maillard Reaction" Kodansha,Ltd.Elsevier, 588 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Ito,; Tadashi Noguchi; Hhiroshi Naito: "Fluorometric Determination of Gizzerosine, a Histamine H_2-Receptor Agonist Discovered in Feedstuffs, Employing High-Performance Liquid Chromatography." Analytical Biochemistry. 151. 28-31 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenji Mori,;Takeshi Sugai,;Yukari Maeda,;Tomomi Okazaki,;Tadashi Noguchi;Hiroshi Naito: "Synthesis of the Racemic and Optically Active Forms of Gizzerosine, an Inducer of Gizzard Erosion in Chicks." Tetrahedron. 41. 5307-5311 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuneo Hino,;Tadashi Noguchi;Hiroshi Naito: "Effect of Gizzerosine on Acid Secretion by Isolated Mucosal Cells of Chicken Proventriculus." Poultry Science. 66. 548-551 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Naito,;Yoshinori Ito;Tadashi Noguchi: Amino-Carbonyl Reaction in Food and Biological Systems---Proceedings of the 3rd International Symposium on the Maillard Reaction.Kodansha-Elsevier, 523-527 (1986)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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