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1986 Fiscal Year Final Research Report Summary

A novel apparatus for measuring the velocity of platelet aggregation, with TV image processing.

Research Project

Project/Area Number 60870003
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionEhime University

Principal Investigator

SHIGA Takeshi  Ehime University, Professor, 医学部, 教授 (10028350)

Co-Investigator(Kenkyū-buntansha) OKAZAKI Masaharu  Ehime University, Research Associate, 医学部, 助手 (20169098)
MAEDA Nobuji  Ehime University, Associate Professor
Project Period (FY) 1985 – 1986
KeywordsPlatelets / Aggregation / Adhesion / Kinetics / 画像処理
Research Abstract

We have made an apparatus (with software) for the quantitative measurement of platelet aggregation and adhesion.
1. The principles of operation are as follows. (1) The reaction chamber is composed of a rotating cone (variable speed) and a plate (e.g., polymethylmethacrylate), and the platelets (mixed with ADP or adrenaline) are poured into the gap between the cone and the plate, in order to give a certain shear rate. (2) The microscopic TV images are separated into two images using a TV image processor: one is the averaged image (of consecutive 8 TV pictures) in which the stationary spots of the adhered platelets are intensified; the other is the image of the moving spots of the flowing platelets which is obtained by subtracting the averaged image from the next TV picture. (3) These images are digtized (1 bit) at a certain grey level, then encoded to the number and the total area of the spots in a frame(real dimension of 190 x 150 <micro> m) using a TV image analyzer. (4) Above processes (2 and 3) are repeated at an interval of 5 sec, and finally the time courses are displayed: e.g., total area of the adhered spots vs. time, total area/spot of the flowing spots vs. time, etc. (5) The size histograms of the observed spots are obtained with another program.
2. The appropriate experimental conditions for human platelet rich plasma are established: i.e., 1 - 3 /s of shear rate, 80 - 200 x 1000 / <micro> l of platelets, 0.2 - 0.5 <micro> M ADP or 1 - 5 <micro> M adrenaline, at 25゜C.
3. The observed time courses of platelet adhesion are successfully simulated by the calculation based on a simple kinetic model, with three rate constants: it is proved that the growth rate of adhered spots is faster than that of de nove adhesion onto the free surface.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Shiga,T.;Maeda,N.;Okazaki,M.: Microcirculation 1986. 21-21 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立石憲彦,岡崎正治,前田信治,志賀健: 日本バイオレオロジー学会論文集. 9. 275-278 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi,,N.;Okazaki,M.;Maeda,N.;Shiga,T.: Biorheology. 23(5). 542-542 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi,N.;Shiga,T.: 1987 International congress on membrane and membrane processes. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立石憲彦,岡崎正治,前田信治,志賀健: 第64回日本生理学会大会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiga,T. et al: "Quantitative measurment of platelet adhesion on foreign surface, studied by TV image analyzer." J. Physiol. Soc. Japan. 47. 574-574 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,N. et al: "A kinetic determination of platelet adhesion onto material surface -Rheoscopic TV image processinf and kinetic model-" Proc. Japan. Soc. Biorheology. 9. 275-278 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiga,T. et al: "A novel quantitative method for the rate measurement of platelet adhesion on material surface - TV image processing of rheoscopy -" Microcirculation 1986. 21-21 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,N. et al: "Determination and kinetics of platelet adhesion onto material surface." Proc. 1987 Internatl. Congress Membrane and Membrane Processes. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,N. et al: "A kinetic determination of platelet adhesion onto material surface -Rheoscopic TV image processing and kinetic model-" Biorheology. 23. 542-542 (1986)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-09  

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