1986 Fiscal Year Final Research Report Summary
Application of monoclonal antibodies directed to carbohydrate antigens to the diagnosis of leukemia
| Project/Area Number |
60870042
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Kyoto University |
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Principal Investigator |
KANNAGT Reiji Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (80161389)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Leukemia / Carbohydrate Antigen / Monoclonal Antibodies / Stem Cell / 蛍光抗体法 / 有機合成 |
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| Research Abstract |
The purpose of this research is to apply monoclonal antibodies directed to carbohydrate antigens into the field of diagnosis of leukemia and other hematological disorders. Normal and pathological blood cells were analyzed by these anti-carbohydrate monoclonal antibodies by various methods. In the first year of this research, blood film samples prepared from either perhipheral blood or bone marrow taken from normal individual and patients with leukemia were analyzed by enzymoimmunohistological techniquies. The results showed that a series of fucosylated polylactosamine antigens is specifically present in the mature and immature granulocyte series of cells, either of normal individuals or of patients. These antigens are useful for the differential diagnosis of lymphatic and non-lymphatic leukemias. In the second year, the distribution of four fucosylated polylactosamine antigens in the colony cells prepared from in various vitro colonies, such as BFU-E, CFU-C, Mega-C, and macrophage colony. The results indicated that BFU-E colony cells contain none of these four polylactosamine antigens, CFU-C contains all four antigens, and Mega-C contain only one species of these antigens, namely fucosyl-SSEA-1 antigen, and macrophage colony cells specifically contain sialyl-SSEA-1 antigen. Several new monoclonal antibodies directed to the carbohydrate antigens in murine leukemia cells were established by using the chemically-synthesized glycolipid antigens in the second year of this investigation.
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[Publications] Kannagi, R., Fukushi, Y., Tachikawa, T., Noda, A., Shin, S., Shigeta, K., et al.: "Quantitative and qualitative characterization of cancer-associated serum glycoprotein antigens expressing fucosyl or sialosy-fucosyl type 2 chain polylactosamine." Cancer Res.46. 2619-2626 (1986)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shigeta, K., Kirihata, Y., Ito, Y., Ogawa, T., Hakomori, S., Kannagi, R.: "Monoclonal antibody directed to a chemically-synthesized cancer-associated glycosphingolipid antigens." J. Biol. Chem.262. 1358-1362 (1986)
Description
「研究成果報告書概要(欧文)」より
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