1986 Fiscal Year Final Research Report Summary
BASIC RESEARCH FOR THE FUSION AND DESTRUCTION OF LIPOSOMES BY PHOTOACTIVATION
| Project/Area Number |
60870089
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般
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| Research Institution | Kyoto University |
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Principal Investigator |
ORII YUTAKA FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 助教授 (60028149)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANISHI SHOSUKE FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 講師 (10025637)
SANO SEIYO FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 教授 (60025533)
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| Project Period (FY) |
1985 – 1986
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| Keywords | FUSION OF LIPOSOMES / DESTRUCTION OF LIPOSOMES / PORPHYRIN LIPOSOMES / DYE LASER / リポソーム破壊 / 光励起 |
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| Research Abstract |
BASIC EXPERIMENTS FOR THE FUSION AND DESTRUCTION OF LIPOSOMES WERE CARRIED OUT AS FOLLOWS.
1. A LARGE SCALE PREPARATION METHOD OF PROTEOLIPOSOMES WAS DEVELOPED BY INTRODUCING THE DIALYSIS PROCEDURE.
2. A DYE LASER FLASH PHOTOLYSIS APPARATUS WAS CONSTRUCTEDINCORPORATING INTERFACES FOR A PERSONAL COMPUTOR. A DEVICE CONTROL AND DATA ANALYSES COMPUTOR PROGRAMS WERE PREPARED BY USING C LANGUAGE.
3. SPECTROPHOTOMETRIC AND ELECTROCHEMICAL PROCEDURES WERE DEVISED TO MONITOR THE DESTRUCTION OF LIPOSOMES.
4. WHEN BOTH BACTERIORHODOPSIN AND CYTOCHROME OXIDASE LIPOSOMES WERE FUSED WITH CALCIUM ION, A RELEASE OF RESPIRATION WAS OBSERVED WHEN IRRADIATED. THIS EXTENT WAS UTILIZED TO MONITOR THE FUSION OF LIPOSOMES.
5. LIPOSOMES RECONSTITUTED WITH PROTOPORPHYRIN WAS DESCTRUCTED RAPIDLY WHEN IRRADIATED, ITS EXTENT BEING DEPENDENT ON THE CONCENTRATION OF OXYGEN IN THE MEDIUM. UNDER NITROGEN THE DESTRUCTION WAS INHIBITED TO A LARGE EXTENT.
6. THE ENZYMIC ACTIVITY OF LIPOSOMES RECONSTITUTED WITH CYTOCHROME OXIDASE AND PROTOPORPHYRIN WAS INHIBITED ALMOST COMPLETELY BY IRRADIATION.
7. PHOTOEXCITATION OF PORPHYRINLIPOSOMES AND ITS DECAY WERE ANALYZED BY THE FLASH-PHOTOLYSIS METHOD REVEALING THE BIPHASIC DECAY OF THE EXCITED STATE. THE DECAY WAS ALSO DEPENDENT ON THE OXYGEN CONCENTRATION.
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