• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1987 Fiscal Year Final Research Report Summary

Production of antisera against various hormones of exprinmental animals.

Research Project

Project/Area Number 60890003
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionGunma University

Principal Investigator

WAKABAYASHI Katsumi  Gunma University, Institute of Endocrinology, 内分泌研究所, 教授 (80008614)

Co-Investigator(Kenkyū-buntansha) TANAKA Shigeyasu  Gunma University, Institute of Endocrinology, 内分泌研究所, 助手 (90146233)
HATTORI Masa-aki  Gunma University, Institute of Endocrinology, 内分泌研究所, 教務員 (60175536)
Project Period (FY) 1985 – 1987
KeywordsANTISERUM / ANTIBODY / MONOCLONAL ANTIBODY / HORMONES / POLYMORPHISM / QUANTITATIVE EXTRACTION OF PITUTARY HORMONES
Research Abstract

With a purpose of distributing antisera to the research scientists in the fields of basic medicine, pharmaceutical sciences, biology, animal husbandry and fishyry, poly clonal and monoclonal antisera were prepared against hormones of various experimental animals with the trials to improve the mothods of immunization. During the research period, polyclonal antisera were produced agains rat PRL, ovine PRL, rat GH, rat TSH, rat LH, oving LH-<beta>, oving TSH-<beta>, ovine GTH-<alpha>, chicken LH, bullfrog LH, bf FSH, hCG, hCG-<beta>, deglycosylated hCG, porcine motilin, human antiotensin-II, renin substrate, melatonin, testosterone, estradiol, cortisol, corticosterone, androstenedione, 17 -progesterone, progesterone, CAMP, rat albumin, rabbit IgG, guinea pig IgG, monkey IgG, and nouse IgG, while monoclonal antibodies were produced against mammalian LHRH, bf GTH-<alpha>, bf LH-<alpha>, bf FSH-<alpha>, ovine LH, ovine FSH, rat PRL, and hoeseradish peroxidase. These antisera have been preliminarily distributed after characterization from May, 1987, and 184 bottles of antisera were supplied to 79 scientists until the end of March, 1988. In connection to the antibody preparation, the quantitative extraction method for pituitary hormoned and polymorphism of pituitary hormones were also studied.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 若林 克己: 生体の科学. 38. 410-412 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Park,M-K.: Endocrinologia Japonica. 33. 257-272 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] park,M-K.: General and Comparative Endocrinology. 68. 82-90 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wadabayashi,K.: Acta Histochemica et Cytochemica. 21. IN PRESS (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa,J.: Endocrinologia Jaonica. 34. 755-767 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozawa,K.: Endocrinologia Japonica. 35. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wakabayashi,K.: "Pars Distalis of the Pituitary Gland - Structure, Function and and Regulation. Biosynthesis and release of luteinizing hormone in relation to hormonal polymorphism." Yoshimura, F., and Gorbman, A. eds. Elsevier Science Publishers, Amsterdam, 549 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Park, M-K.: "Preparation of a monoclonal antibody to common amino acid sequence of LHRH and its application." Endoctinologia Japonica. 33. 257-272 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Park, M-K.: "Production and characterization of a monoclonal antibody against the <beta>-subunis of bullfrog lutropin." General and Comparative Endocrinology. 68. 82-90 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wakabayashi, K.: "Assessment of specificity of antisera for immunohistochemistry." Acta Histochemica et Cytochemica. 21. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, J.: "Choice of extraction procedure for estimation of anterior pituitary hormone content." Endocrinologia Japonica. 34. 755-767 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozawa, K.: "Dynamic change in charge heterogeneity of pituitary FSH through the estrous cycle in demale rats." Encodrinologia Japonica. 35. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wakabahashi, K.: Elsevier Science Publishers, Amsterdam. Biosynthesis and release of Luteinizing hormone in relation to hormonal polymorphism. in "pars Distalis of the Pitutary Gland", 363-369 (1986)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1989-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi