Regulatory mechanisms of structure and function of muscle

1988 Fiscal Year Final Research Report Summary

• Project/Area Number: 61304068
• Research Category: Grant-in-Aid for Co-operative Research (A)
• Allocation Type: Single-year Grants
• Research Field: 分子遺伝学・分子生理学
• Research Institution: Kyushu University
• Principal Investigator: OHTSUKI Iwao Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (70009992)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Koui Hokkaido University, Faculty of Agriculture, Assoc. Professor, 農学部, 助教授 (40001432) ; MASAKI Tomoo Tsukuba University, Faculty of Medicine, Professor, 基礎医学系, 教授 (60009991) ; YAMADA Kazuhiro Ooita Medical University, Professor, 教授 (20053027) ; OBINATA Takashi Chiba University, Faculty of Science, Professor, 理学部, 教授 (40012413) ; MARUYAMA Koscack Chiba University, Faculty of Science, Professor, 理学部, 教授 (60012267)
• Project Period (FY): 1986 – 1988
• Keywords: Muscle / Muscle contraction / Calcium regulatory proteins / Cytoskeletal proteins / Skeletal muscle / 平滑筋

Research Abstract

Structure and function of regulatory and cytoskeletal proteins of muscle were investigated with special reference to the role at physiological conditions as follows.
(1) Ca-regulatory proteins of striated muscle. CDTA-treated myofibrils was proved to be suitable for the research on the physiological role of troponin C in contractile response. Binding of four Ca^<2+> ions to troponin C molecule in the thin filament was found necessary for the activation of myofibrillar ATPase activity. Calorimetry of frog skeletal troponin C was carried out.
(2) Cytoskeletal proteins. Localization of connectin in relation to striation patterns was investigated by immunoelectron microscopy. It was found that connectin in A band is less extensible than that in I-band. Connectin filament itself was visualized electron microscopically in the myofibrils treated with gelsolin. Preparative procedures for paratropomyosin was also investigated.
(3) Structural regulatory proteins. Primary structure of -actinin molecules from smooth muscle and embryonic skeletal muscle was determined by the examining cDNA. Regulatory proteins of actin polymerization in chick embryonic muscle was investigated.
(4) Regulatory proteins of smooth muscle. Preparative procedure of natural actomyosin and the method for desensitization of natural actomyosin have been established successfully. Using this protein preparation properties of Ca^<2+> sensitizing protein such as leiotonin were investigated. Study was carried out on the proteins regulating the length of actin filament.

Research Products

• [Publications] Ohtsuki,I.;Yamamoto,K.: Bioactive Molecules. 8. 65-70 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato,H.;Fukunaga,K.;Araki,S.;Ohtsuki,I.;Miyamoto,E.: Arch.Biochem.Biophys.260. 443-451 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohtsuki,I.;Onoyama,Y.;Shiraishi,F.: J.Biochem.103. 913-919 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Morimoto,S.;Ohtsuki,I.: J.Biochem.104. 149-154 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Morimoto,S.;Fujiwara,T.;Ohtsuki,I.: J.Biochem.104. 873-874 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Morimoto,S.;Ohtsuki,I.: J.Biochem.105. 730-734 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shiraishi,S.;Ohtsuki,I.: Fukuoka Acta Med.submitted. (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Itoh,Y.et al.: J.Biochem.104. 504-508 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shimizu,T.et al.: Biochem.Res.9. 227-233 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hu,P.H.;Kimura,S.;Maruyama,K.: J.Biochem.104. 504-511 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Higuchi,H.;Yoshioka,T.;Maruyama,K.: J.Muscle Res.Cell Motility. 9. 491-498 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ebashi,S.Ogawa,Y.: Handbook of experimental Pharmacology. 83. 31-56 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ebashi,S.;Ogawa,Y.: Biophys.Chemi.29. 137-143 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ebashi,S.;Koga,R.: Proc.Japan Academy. 64B(4). 98-101 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kuwayama,H.;Ebashi,S.: J.Biochem.104. 858-861 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kuwayama,H.;Suzuki,M.;Koga,R.;Ebashi,S.: J.Biochem.104. 862-866 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iahizaki,Y.;Mikawa,T.;Ebashi,S.;Yokota,E.;Hosaya,H.;Kuroda,K.: J.Biochem.104. 329-332 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ebashi,S.;Koga,R.: Proc.Japan Academy. 64(B)10. 323-326 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oobatake,M.;Ooi,T.: J.Biochem.104. 433-439 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ooi,T.;Oobatake,M.: J.Biochem.104. 440-444 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakashima,H.;Nishikawa,K.;Ooi,T.: J.Protein Chem.7. 509-525 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Imaizumi,M.;Tanokura,M.;Yamada,K.: J.Physiol.Soc.Japan. 50. 483 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 田之倉優、今泉雅資、山田和広: 蛋白質・核酸・酵素. 臨時増刊. 2003-2012 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawano.Y.;Tanokura,M.;Yamada,K.: J.Physiol.407. 243-262 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamada,K.Tanokura,M.: Proc.Jpn.Acad. 64. 278-286 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamada,K.Tanokura,M.: Proc.Jpn.Acad.64. 278-286 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamada,K.Tanokura,M.: Proc.Jpn.Acad.64. 287-289 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Imamura;T.Endo;M.Kuroda;T.Tanaka;T.Masaki: J.Biol.Chem.263. 7800-7805 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Itoh;M.Yanagisawa;S.Ohkubo;C.Kimura;T.Kosaka;A.Inoue;N.Ishida;Y.Mitsui.: FEBS Lett.231. 441-444 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Yanagisawa;A.Inoue;T.Ishikawa;Y.Kasuya;S.Kimura;S.Kumagaya;K.Nakajima;T.X.Watanabe;S.Sakakibara;K.Goto;T.Masaki: Proc.Nat.Acad.Sci. 85. 6964-6967 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Ishikawa.;N.Okamura;A Saito;T.Masaki;K.Goto: Circ.Res.63. 726-734 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Ishikawa;M.Yanagisawa;S.Kimura;K.Goto;T.Masaki;: Am.J.Physiol.255. H970-973 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] C.Arimura;T.Suzuki;M.Yanagisawa;M.Imamura;Y.Hamada;T.Masaki: Eur.J.Biochem.177. 649-655 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takano-Ohmuro,H.;Goldfine,S.M.;Kojima,T.;Obinata,T.;Fischman,D.A.: J.Muscle Res.Cell Motility. (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oshima,S.;Komiya,T.;Takeuchi,K.;Endo,T.;Obinata,T.: Comparative Biochemistry and Physiology. 90B. 779-784 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takagi,M.;Takano-Ohmuro H.;Nakamura,T.;Kawahara;H.,Shimizu;T.Obinata,T.;Kohama,K.: Muscle and Nerve. (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Abe,H.;Obinata,T.: Cellular and Molecular Biology of Muscle Development. (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Obinata,T.;Ogasawara,Y.Takeuchi,K.;Takano-Ohmuro,H.: Etiology and Morphogenesis of Cogenital Heart Disease. (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 大日方昂: 蛋白質・核酸・酵素. 33. 2033-2042 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kurokawa,H.;Nonomura,Y.: J.Biochem.103. 8-10 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakanishi,S.;Yamada,K.;Kase,H.;Nakamura,S.;Nonomura,Y.: J.Biol.Chem.263. 6215-6219 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura,S.;Ohmi,K.;Nonomura,Y.: Molecul.Pharmacol.33. 604-610 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura,S.;Nabeshima,Y.;Kobayashi,H.;Nabeshima,Yoko;Nonomura,Y.;Fujii-Kuriyama,Y.: J.Mol.Biol.203. 895-904 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagano,K.;Iinuma,I.: J.Prot.Chem.7(3). 268-269 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagano,K.;Michal,H.;Takezawa,M.: J.Theor.Biol.134. 199-256 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori,A: J.Biochem.103. 809-814 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamanoue,M.: J.Biochem.103. 843-847 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 高橋興威: 生物科学. 40. 140-147 (1988)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takahashi,M.: J.Biochem.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takahashi,K.: J.Biochem.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 長野晃三: "続生化学実験講座" 東京科学同人, 198 (1987)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohtsuki, I.; Yamamoto, K.: "Troponin and other calcium binding proteins of muscle." Bioactive Molecules. 8. 65-70 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato, H.; Fukunaga, K.; Araki, S.; Ohtsuki, I.; Miyamoto, E.: "Identification of the multifunctional calmodulin-dependent protein kinase in the cytosol, sarcoplasmic reticulum, and sarcolemma of rabbitskeletal muscle." Arch. Biochem. Biophys.260. 443-451 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohtsuki, I.; Onoyama, Y.; Shiraishi, F.: "Electron microscopic study of troponin." J. Biochem.103. 913-919 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Morimoto, S.; Ohtsuki, I.: "Effect of substitution of troponin C in cardiac myofibrils with skeletal troponin C or calmodulin on the Ca^<2+>- and Sr^<2+>-sensitive ATPase activety." J. Biochem.104. 149-154 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Morimoto, S.; Fujiwara, T.; Ohtsuki, I.: "Restoration of Ca^<2+>-activated tension of CDTA-treated single skeletal muscle fibers by troponin C." J. Biochem.104. 873-874 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Morimoto, S.; Ohtsuki, I.: "Ca-binding to skeletal muscle troponin C in skeletal and cardiac myofibrils." J. Biochem.105. 730-734 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shiraishi, S.; Ohtsuki, I.: "Removal of troponin C from skeletal natural actomyosin by CDTA-treatment." Fukuoka Acta Med.(1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Itoh, Y., et al.: "Extensible and less extensible domains of connectin filaments in stretched skeletal muscle fibers." J. Biochem.104. 504-508 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimizu, T., et al.: "An immunological homology between neurofilament and elastic filament." Biochem. Res.9. 227-233 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hu, P. H.; Kimura, S.; Maruyama, K.: "Crosslinking of native myosin froms oligomers of myosin heavy chains." J. Biochem.104. 504-511 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Higuchi, H.; Yoshioka, T.; Maruyama, K.: "Positioning of actin filaments in skinned muscle fibers released after stretch." J. Muscle Res. Cell Motility. 9. 491-498 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ebashi, S.; Ogawa, Y.: "Troponin C and calmodulin as calcium receptors: Mode of actin and sensitivity to drugs." Handbook of experimental Pharmacology. 83. 31-56 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ebashi, S.; Ogawa, Y.: "Ca^<2+> in contractile processes." Biophys. Chemi.29. 137-143 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ebashi, S.; Koga, R.: "Desensitization of smooth muscle natural actomyosin." Proc. Japan Academy. 64B(4). 98-101 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuwayama, H.; Ebashi, S.: "Isolation from bovine brain of 155 kDa component exhibiting myosin ligh chain Kinase activity." J. Biochem.104. 858-861 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuwayama, H.; Suzuki, M.; Koga, R.; Ebashi, S.: "Preparation of protein components exhibiting myosin light chain kinase activities from bovine aorta: Discrepancies between its enzyme activity and actomyosin activating effect." J. Biochem.104. 862-866 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iahizaki, Y.; Mikawa, T.; Ebashi, S.; Yokota, E.; Hosaya, H.; Kuroda, K.: "Preparation of tubulin from caulerpa, a marine green alga, using casein as a protectove agent against proteolytic degredation." J. Biochem.104. 329-332 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ebashi, S.; Koga, R.: "The use of dimethyl sulfoxide (DMSO) for protein fractionation." Proc. Japan Academy. 64(B)10. 323-326 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oobatake, M.; Ooi, T.: "Characteristic thermodynamic properties of hydrated water for 20 amino acid residues in globular proteins." J. Biochem.104. 433-439 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ooi, T.; Oobatake, M.: "Intermolecular interactions between protein and other molecules including hydration effects." J. Biochem.104. 440-444 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakashima, H.; Nishikawa, K.; Ooi, T.: "Segmentation of a protein into strutural elemets: Analysis and classification of segments." J. Protein Chem.7. 509-525 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Imaizumi, M.; Tanokura, M.; Yamada, K.: "Calcium and magnesium binding to troponin C from bullfrog skeletal muscle." J. Physiol. Soc. Japan. 50. 483 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kawano, Y.; Tanokura, M.; Yamada, K.: "Phosphorus nuclear magnetic resonance studies on the effect of duration of contraction in bull-frog skeletal muscles." J. Physiol.407. 243-262 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamada, K.; Tanokura, M.: "Time-resolved ^<31>P-NMR studies on contraction and recovery of bullfrog sartorius muscles. An inproved system for physiological measurements." Proc. Jpn. Acad. 64. 278-286 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamada, K.; Tanikura, M.: "Time-resolved ^<31>P-NMR studies on contraction and recovery of bullfrog sartorius muscles. A post-contractile increase in NMR visible phosphates." Proc. Jpn. Acad. 64. 287-286 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamada, K.; Tanokura, M.: "Time-resolved ^<31>P-NMR studies on contraction and recovery of bullfrog sartorium muscles. Mechanism of post-contractile increase in NMR visible phosphates." Proc. Jpn. Acad. 64. 287-289 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Imamura, M.; Endo, T.; Kuroda, M.; Tanaka, T.; Masaki, T.: "Substructure and higher structure of chicken smooth muscle -actinin molecule." J. Biol. Chem.263. 7800-7805 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Itoh, Y.,;Yanagizawa, M.,;Ohkubo, S.,;Kimura, C.,;Kosaka, T.,;Inoue, A.,;Ishida, N.,;Mitsui, Y.;Onda, H.,;Fujino, M.; Masaki, T.: "Cloning and seguence analysis of cDNA encoding the precursor of a human endothelium derived vasoconstrictor peptide, endothelim identity of human and porcine endothelin." FEBS Lett.231. 441-444 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yanagizawa, M.,;Inoue, A.,;Kshikawa, T.,;Kasuya, Y.,;Kimura, S.,;Kumagaya, S.,;Nakajima, K.,;Watanabe, T.X.,;Sakakibara, S.,;Goto, K.;Masaki, T.: "Primary structure, synthesis and biological activity of rat endothelin, an endothelium derived vasoconstrictor peptide." Proc. Nat. Acad. Soi.85. 6964-6967 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishikawa, T.; Okamura, N.; Saito, A.; Masaki, T.; Goto, K.: "Positive inotropic effect of calcitonin gene related peptide (CGRP) mediated by cyclic AMP in the guinea pig heart." Circ, Res.63. 726-734 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishikawa, T.; Yanagizawa, S.; Kinura, K.; Goto, K.; Masaki, T.: "The positive inotropic action of a novel endothelium derived vasoconstrictor peptide, endothelin, on guinea pig atria." Am. J. Physiol.255. 970-973 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Arimura, C.; Suzuki, T.; Yanagizawa, M.; Imamura, M.; Hamada, Y.; Masaki, T.: "Primary structure of chicken skeletal muscle and fibroblast -actinins deduced from cDNA seguences." Eur. J. Biochem.177. 649-655 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takano-Ohmuro, H.; Goldfine, S.M.; Kojima, T.; Obinata, T.; Fischman, D.A.: "Size and charge heterogeneity of C-protein isoforms in avian skeletal muscle. Expression of six different isoforms in developing chicken pectoralis muscle." J. Muscle res. Cell Motility. In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oshima, S.; Komiya, T.; Kakeuchi, K.; Endo, T.; Obinata, T.: "Generation of multiple troponin T isoflrms is a common feature of the muscles in various chordate animals." Comparative Biochemistry and Physiology. 90B. 779-784 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takagi, M.; Takano-Ohmuro, H.; Nakamura, T.; Kawahara, H.; Shimizu, T.; Obinata, T.; Kohama, K.: "Cross-reactivity of an anti-neurofilament antibody with a troponin isoform." Muscle and Nerve. In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Abe, H.; Obinata, T.: "Regulation of actin assembly in developing skeletal muscle: isolation of two actin regulatory proteins from embryonic chick skeletal muscle." Cellular and Molecular Biology of Muscle Development. In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Obinata, T.; Ogawawara, Y.; Takeuchi, K.; Takano-Ohmuro, H.: "Troponin T and myosin light chain isoforms which characterize embryonic chicken cardiac muscle." Etiology and Morphogenesis of Cogenital Heart Disease. In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kurokawa, H.; Nonomura, Y.: "Purification of a novel 30、000 Da calcium-binding protein from bovine cerebellum." J. Biochem.103. 8-10 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakanishi, S.; Yamada, K.; Kasa, H.; Nakamura, S.; Nonomura, Y.: "K-252a, a novel microbical produce, inhibits smooth muscle myosin ligh chain kinase." J. Biol. Chem.263. 6215-6219 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura, S.; Ohmi, K.; Nonomura, Y.: "Low concentration of reserpine accelerates actin polymerization via with G-actin." Molecul Pharmacol.33. 604-610 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamara, S.; Nabeshima, Y.; Kobayashi, H.; Nabeshima, Yoko,; Nonomura, Y.; Fujii-Kuriyama, Y.: "Single chicken cardiac myosin alkali light-chain gene generates two different mRNAs by alternative splicing of a complex exon." J. Mol. Biol.203. 895-904 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagano, K.; Iinuma, I.: "Membrane controlled folding mechanism of hepatitis B surface anligens of human, woodchusk, ground squirrel and duck." J. Prot. Chem.7(3). 268-269 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagano, K.; Michal, H.; Takezawa, M.: "Prediction of three-dimensional structure of escherichia coli ribosomal RNA" J. Theor. Biol.134. 199-256 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori, A.: "Localization of paratropomyosin in skeletal muscle myofibrils and its translocation during postmortem storage of muscle." J. Biochem.103. 809-814 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamanoue, M.: "Effect of paratropomyosin on the increase in sarcomere length of rigor-shortened skeletal muscle." J. Biochem.103. 843-847 (1988)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takahashi, M.: "Ca^<2+>-sensitive transtion in the conformation of molluscan myosin molecules." J. Biochem.In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takahashi, M.: " -actinin is a component of the Z filament, a structural backbone of skeletal muscle Z-disks." J. Biochem.In press. (1989)
  • Description: 「研究成果報告書概要(欧文)」より