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1988 Fiscal Year Final Research Report Summary

Structure and Function of the plasmid genes associated with epethelial invasion in Shigella Flexneri

Research Project

Project/Area Number 61440035
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionUniversity of Tokyo

Principal Investigator

YOSHIKAWA Masanosuke  Institute of Medical Science, Univ. of Tokyo, 医科学研究所, 教授 (80012714)

Co-Investigator(Kenkyū-buntansha) MAKINO Sou-ichi  Institute of Medical Science, Univ. of Tokyo, 医科学研究所, 助手 (30181621)
SASAKAWA Chihiro  Institute of Medical Science, Univ. of Tokyo, 医科学研究所, 助教授 (70114494)
Project Period (FY) 1986 – 1988
KeywordsPathogenicity / Virulence / tolic Shigella / Cell invasion / Plasmid / Recombinant DNA technology / Molecular genetics / 転写アクチベータ
Research Abstract

Bacillary dysentery is an invasive infectious diseases of the human colon. A 230-kb plasmid as well as genetic loci on the chromosome have been implicated in its pathogenesis. Results obtained by the molecular genetic analysis mainly of the plasmid genes, virG, virF, and virB and on a chromosome gene kcpA are summarized as follows. 1. The SalI restriction map of the plasmid, pMYSH6000 of S. flexneri 2a was made. Tn5 insertions into pMYSH6000 were isolated, and the sites of Tn5 assigned to each of the 23 SalI fragments. Some insertions into SalI-G, SalI-F and 4 contiguous SalI fragments, B-P-H-D resulted in avirulence. The Tn5 insertions into B-P-H-D defined 5 separable regions, tentatively designated as Regions 1 through 5. A single cistron virG consisting of 3306 bp and encoding a 116 kd protein required for intercellular spread has been defined within SalI-G. A cistron virF on SalI-F fragment has been identified and sequenced. Expression of 4 immunodominat proteins, one encoded by virg, and 3 encoded by Region 2 were found to be positively regulated by the 30 kD virF protein at the transcriptional level. This occurred directly for the virG but, for 3 Region 2 genes, exclusively via transcriptional activation by a 33 kd protein activator encoded by another cistron virB on Region 1. 2. A chromosomal locus kcpA containing a single cistron has been cloned and the nucleotide sequence determined. A 12.3 kd product also required for intercellular spread has been identified. The complete understanding of the pathogenesis of bacillary dysentery at the molecular level still remains far in the distance. It is our hope that these approaches may facilitate us in reaching the final goal.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Makino S.et al.: Cell. 46. 551-555 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasakawa C.et al.: Infection and Immunity. 54. 32-36 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai T.et al.: Infection and Immunity. 54. 395-402 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasakawa C.et al.: J.Bacteriologg. 170. 2480-2484 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino S.et al.: Microbial Pathogenesis. 5. 264-274 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai T.et al.: Molec.Microbiol.2. 589-597 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lett,M.-C.et. al: J.Bacteriology. 171. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,M.et al.: Molec.Microbiol.3. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adler,B.et al.: Molec.Microbiol.3. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikawa,M.et al.: Microbiol.Sciences(IUMS Journal). 5. 333-339 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川昌之介: 日本細菌学会雑誌. 43. 641-652 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川昌之介: 日本細菌学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川昌之介: "感染:病院とその予防ー阿蘇シンポジウム1986ー(赤痢菌の細胞侵入性に関わるプラスミド)" 南山堂, (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikawa,M. et al.: "Advances in Research on Cholera and Related Diarrheos 6巻" KTK Scientific Publishers(日米医学協力コレラ部会), (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笹川千尋: "遺伝子からみた細菌の病原性 「B群赤痢菌のビルレンス因子」" 菜根出版, (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikawa,M.et al.: "Advances in Research on Cholera and Related Diarrheas 8巻" KTK Scientific Publications(日米医学協力:コレラ部会),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino.S.;et al: "A genetic determinant required for continuous reinfection of adjacent celle on large plasmid in S.flexneri" Cell. 46. 551-555 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasakawa C.;et al: "Isolation, characterization, and mapping of Tn5 insertions into the 140-megadalton invasion plasmid defective in mouse Sereny test in Shigella Flexneni 2a" Infection and Immunity. 54. 32-36 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai T.;et al: "DNA sequence and product analysis:of the virF locus responsible for Congo red binding and cell invasion in Shigella Flexneri 2a" Infection and Immunity. 54. 395-402 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasakawa C.;et al: "Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella Flexneri 2a" J. Bacteriology. 170. 2480-2484 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino S.;et al: "Genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive Escherichia coli" Microbial Pathogenesis. 5. 264-274 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai.T.;et al.: "Expression of four virulence antigens of Shigella Flexneri is positively regulated at the transcriptional level by the 30 kilodalton virF protein" Molec. Microbiol.2. 589-597 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lett, M.-C.; et al: "virG, a Plasmid-coded virulence gene of Shigella Flexneri : Identification of the virG protein and determination of the complete coding sequence" J. Bacteriology.171. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, M.;et al.: "Molecular cloning and characterization of chromosomal virulence region KCPA of Shigella Flexneri" Molec. Microbiol.3. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adler, B.;et al.: "A dual transcriptional activasion system for the 230 Kb plasmid genes coding for virulence-associated antigens of Shigella Flexneri" Molec. Microbiol.3. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikawa, M.;et al.: "Molecular genetic approaches to the pathogenesis of bacillary dysentery" Microbiol. Sciences (IUMS Journal). 5. 333-339 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikawa, M.;et al: Advances in Research on Cholera and Related Diarrheas. KTK Scientific Publishers, (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikawa, M.;et al: Advances in Research on Cholera and Related Diarrheas. KTK Scientific Publications, 8

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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