細胞間情報伝達の遺伝子機構と免疫ネットワ-ク・システムの形成に関する研究

1989 Fiscal Year Annual Research Report

• Project/Area Number: 61440036
• Research Institution: Chiba University
• Principal Investigator: 谷口 克 千葉大学, 医学部, 教授 (80110310)
• Co-Investigator(Kenkyū-buntansha): 田川 雅敏 千葉大学, 医学部, 助手 (20171572) ; 徳久 剛史 神戸大学, 医学部, 教授 (20134364)
• Keywords: 抑制T細胞 / Vα14α鎖遺伝子 / 多様性欠除 / 自己反応性レパトア / 胸腺非依存性の選択 / 分子生物学 / 免疫系ネットワ-ク

Research Abstract

KLH抑制T細胞抗原レセプタ-α鎖遺伝子の解析を行い次の結果を得た。1.新しいTCR遺伝子ファミリ-(Vα14)を発見した。2.Vα14を遺伝子プロ-ブにしたサザンブロット解析から、ラボマウスは3種類に分類できる。そのうちC57BL系はアジア型野性マウスタイプと、その他のラボマウスはヨ-ロッパ型野性マウスタイプを示した。3.Vα陽性α鎖は正常マウスにおいても90%以上がJα281と結合し、VJ結合はほとんど1塩基挿入のN領域(グリシン)で構成されるhomogenous receptorであり、positive selectionの結果であると考えられた。4.V14J281α鎖は全α鎖の1.5%をも占め、高頻度に発現していた。5.このリガンドはキメラマウスの実験から未知の自己成分でマウス亜種特異的に発現する分子であることが判明した。6.このα鎖の選択は胸腺非依存性にもおこなわれていることが、ヌ-ドマウスや胸腺摘出X線照射骨髄マウスを用いた実験から示唆された。以上のことから、抑制T細胞は自己反応性レパトアに属し、自己寛容の維持に重要な働きを持つと考えられた。

Research Products

• [Publications] Taniguchi,M.,Imai,K.,Matsushita,E.,Sumida,T.,Tagawa,M.,Yamamoto,S.and Kanno,M.: "Antigenーreceptor and functional molecule of suppressor T cell." SinoーJapan J.of Allergol.Immunol.3. 225-229 (1986)
• [Publications] Imai,K.,Kanno,M.,Sigemoto,K.and Taniguchi,M.: "Sequence and expression of transcripts of the T cell receptor alphaーchain in a gene functional,antigen specific suppresoor T cell." Proc.Nat1.Acad.Sci.USA. 83. 8708-8712 (1986)
• [Publications] Shigemoto,K.,Kanno,M.,Sakamoto,T.,Imai,K.and Taniguchi,M.: "Application and limitation of differential hybridization and T cellーspecific cDNA clones." Microbiol.Immunol.31. 899-909 (1987)
• [Publications] Takemori,T.,Miyazoe,I.,Shirasawa,T.,Taniguchi,M.and Graf,T.: "A temperatureーsensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells" EMBO J.4. 951-956 (1987)
• [Publications] Ono,K.,Kato,F.and Taniguchi,M.: "Possible involvement of Lyー1 B cells in the effector phase of IgG suppression mediated by suppresor T cell factor." J.Mol.Cell.Immunol.3. 167-175 (1987)
• [Publications] Takahashi,K.,Ono,K.,Hirabayashi,Y.and Taniguchi,M: "Escape mechanisms of melanoma from immune system by soluble melanoma antigen" J.Immunol.140. 3244-3248 (1988)
• [Publications] Soejima,T.,Nagayama,A.,Sado,T.and Taniguchi,M.: "Acquisition of repertoires of suppressor T cells under the influence of macrophages" J.Mol.Cell Immunol.4. 87-95 (1988)
• [Publications] Koseki,H.,Imai,K.,Ichikawa,T.,Hayata,I.and Taniguchi,M.: "Predominant use of a particular alphaーchain in suppressor T cell hybridomas specific for keyhole limpet hemocyanin." Intl.Immunol.6. 557-564 (1989)
• [Publications] Kiesel,U.,Oschilewski,M.,Taniguchi,M.and Kolb,H.: "Modulation of lowーdose streptozotocinーinduced diabetes in mice by administration of antibodies to IーA,IーE and IーJ determinants" Diabetologia. 32. 173-176 (1989)
• [Publications] Kameyama,K.,Imai,K.,Itoh,T.,Taniguchi,M.,Miura,K.and Kurosawa,Y.: "Convenient plasmid vectors for construction of chimeric mouse/human antibodies" FEBS Lett.244. 301-306 (1989)
• [Publications] Hatano,M.,Aizawa,S.,Soejima,T.,Shigemoto,K.,Taniguchi,M.and Tokuhisa,T.: "Specific inhibition of class II MHC gene expression by antiーsense RNA." Intl.Immunol.1. 260-266 (1989)
• [Publications] Kimoto,H.,Shirasawa,T.,Taniguchi,M.and Takemori,T.: "B cell precursors are present in the thymus during early development" Eur.J.Immunol.19. 97-104 (1989)
• [Publications] Taniguchi,M.: "Suppressor T cells belong to the self reactive repertoire" Res.Immunol. 140. 391-398 (1989)
• [Publications] Koseki,H.,Imai,K.,Nakayama,F.,Sado,T.and Taniguchi,M.: "Homogenous junctional sequence of Valpha14^+ alphaーchain antigen receptor of T cells expanded in unprimed mice." Proc.Natl.Acad.Sci.USA.
• [Publications] Taniguchi,M.and Tokuhisa,T.: "Antigenーspecific suppressor molecules produced by T cells.In:Handーbook of Experimental Immunology(2)." Blackwell, 80 (1986)
• [Publications] Taniguchi,M.,Sumida,T,.Imai,K.,Kanno,M.and Shigemoto,K.: "Antiーidiotypic suppressor T cells that express a stereocopy of the modified suppressor epitope on the nominal antigen.In:Immunogenicity of Protein Antigens:Repertoire and Regulation." CRC Press, 7 (1987)
• [Publications] Imai,K.,Sumida,T.,Kanno,M.,Shigemoto,K.and Taniguchi,M.: "Functional and molecular properties of antiーidiotypic receptor on suppressor T cell ーExpression of alpha chain gene in Ts hybridomaーIn:New Approach to Autoimmune diseases." Academic Press,Tokyo., 8 (1987)
• [Publications] Taniguchi,M.: "Genetic assciation of the suppressor factor that regulate immune respons.In:Antigenーspecific T Cell Receptors and Factors." CRC Press,New York, 12 (1987)
• [Publications] Taniguchi,M.,Matsushita,E.,Imai,K.,Koseki,H.,Sumida,T.,Takemori,T.,Itoh,T.,Yamamoto,S.and Kanno,M.: "Suppressor T cell receptor and functional molecule.In:Molecular Basis of Lymphokine Action." The Humana Press, 8 (1987)
• [Publications] Taniguchi,M.,Imai,K.and Koseki,H.: "Molecular analysis of suppressor cell receptors.In:T cell Receptor Structure." Harwood Academic Publishers, 11 (1988)
• [Publications] Taniguchi,M.: "Is there a unique language in the immunoregulatory system? In:NATO ASI Seris,Vol.H23.The Semiotics of Cellular Communication in the Immune System." SpringerーVerlag, 13 (1988)
• [Publications] Taniguchi,M.,Imai,K.and Koseki,H.: "Predominant use of the particular V gene in the KLH specific suppressor T cell family.In:Theoretical Immunology,Part 2,SFI Studies in the Science of Complexity." AddtionーWesley Publishing, 7 (1988)