• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1989 Fiscal Year Final Research Report Summary

Molecular biological, neurochemical and immunohistochemical studies of the senile dementia-Alzheimer's disease brains.

Research Project

Project/Area Number 61440049
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionPsychiatric Research Institute of Tokyo (PRIT)

Principal Investigator

ISHII Tsuyoshi  PRIT, Dept. of Ultrastructure, Director, 超微形態研究室, 所長 (80090405)

Co-Investigator(Kenkyū-buntansha) KAMETANI Fuyuki  PRIT, Dept. of Molecular Biology, Researcher, 分子生物学研究室, 研究員 (70186013)
TOKUTAKE Sotoshi  PRIT, Dept. of Molecular Biology, Researcher, 分子生物学研究室, 主任研究員 (50090402)
SHINODA Tomotaka  Tokyo Metropolitan Univ. Dept. of Chemistry, Faculty of Science, Associate profe, 理学部化学教室, 助教授 (50000253)
OYANAGI Shinsaku  PRIT, Dept. of Ultrastructure, Chief, 超微形態研究室, 参事研究員 (50018595)
Project Period (FY) 1986 – 1989
KeywordsAlzheimer's disease / Senile plaque / amyloid / amyloid precursor protein / microglial cell / immunoglobulins
Research Abstract

The deposition of amyloid is considered to be one of the most characteristic chemical and pathological hallmarks in the Alzheimer (AD) or Down syndrome (DS) brains. To identify subsequences of amyloid precursor protein (APP) in the AD and control brains, western blots and immunohistochemistry were performed using 6 antibodies against synthetic peptides (subsequences of APP; R35, residues 274-286; R36, residues 527-540; R37, residues 681-695; beta protein, residues 597-620; protein inhibitor domains (PID), residues 325-337, 351-364 (Kitaguchi et al., 1988) on cryostat sections of cerebral cortex and hippocampus of the AD brain and of age-matched controls. Western blots detected all of these peptides, except for PID, in AD as well as in control brains. However, immunohistochemistry could detect only R36 and beta protein in amyloid and R37 (C-terminal of APP) in what may be degenerated neurites in the periphery of senile plaques.
A sensitive methenamine silver/Nissl stain disclosed that pr … More eamyloid were found immediately adjacent to, or around the cell bodies of neurons. We consider an early stage of senile plaque formation to be the deposition of preamyloid substance adjacent to the cell body of neurons. We suggest that the amyloid progressively accumulates around the cell body until the enclosed neuron degenerates.
A monoclonal antibody, termed AD11/8 which is reactive to microglial cells, stains numerous microglial cells intensively and they often formed clusters in gray matter in AD brain. By double immunostaining with AD11/8 and a polyclonal antibody against synthetic amyloid beta protein, clustered microglial cells were observed. in and around senile plaques with amyloid deposits. These results indicate that microglial cells may play an important role in senile plaque formation.
A monoclonal antibody HY20 which stains amyloid deposits in the AD brain, specifically reacted with the variable region of a lambda light chain of human immunoglobulins. It is concluded that an epitope shared with a lambda light chain is included in or associated with amyloid deposits in the AD brain.
These data suggest that an immunological mechanism is involved in the amyloid formation in senile plaques in AD brain. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ishii T,Kametani F,Haga S,and Satoh M: "The immunohistochemical demonstration of subsequences of the precursor of the amyloid A4 protein in senile plaques in Alzheimer brain" Neuropathol & Appl Neurobiol. 15. 135-147 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haga S,Akai K and Ishii T: "Demonstration of microglial-cells in and around senile(neuritic)plaques in the Alzheimer brain-An immunohistochimical study using a novel monoclonal antibody" Acta Neuropathol. 77. 569-575 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iseki E,Matsushita M,Kosaka K,Kondo H,Ishii T and Amano N: "Distribution and morphology of brain stem plaques in Alzheimer's disease" Acta Neuropathol. 78. 131-136 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kametani F,Tanaka K,Haga S,Satoh M,Allsop D and Ishii T: "Demonstration of a 72K β-protein precursor fragment in Alzheimer and normal aged brain." Biomedical Research. 10. 179-183 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Allsop D,Haga S,Haga C,Ikeda S,Mann DMA and Ishii T: "Early senile plaques in Down's syndrome brains show a close relationship with cell bodies of neurons." Neuropathool & Appl Neurobio. 15. 531-542 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Allsop D,Haga S,Bruton C,Ishii T and Roberts GW: "Neurofibrillary tangles in some cases of dementia pugilistica share antigens with amyloid β-protein of Alzheimers's disease." Amer J Pathol. 136. 255-260 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小坂憲司、石井毅: "老化性痴呆と拡痴呆薬" 日本科学技術協会.東京, (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石井毅: "アルツハイマ-型痴呆の正体49ー62頁 朝長正徳編「脳は老化するか」" (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii T, Kametani F, Haga S, and Satoh M: "The immunohistochemical demonstration of subsequences of the precursor of the amyloid A4 protein in senile plaques in Alzheimer brain" Neuropathol & Appl Neurobiol. 15. 135-147 ((1989))

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haga S, Akai K and Ishii T: "Demonstration of microglial cells in and around senile (neurotic) plaques in the Alzheimer brain-An immunohistochemical study using a novel monoclonal antibody" Acta Neuropathol 77, 569-575 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iseki E, Matsushita M, Kosaka K, Kondo H, Ishii T and Amano N: "Distribution and morphology of brain stem plaques in Alzheimer's disease" Acta Neuropathol 78, 131-136 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kametani F, Tanaka K, Haga S, Satoh M, Allsop D and Ishii T: "Demonstration of a 72K beta-protein precursor fragment in Alzheimer and normal aged brain." Biomedical Research 10, 179-183 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Allsop D, Haga S, Haga C, Ikeda S, Mann DMA and Ishii T: "Early senile plaques in Down's syndrome brains show a close relationship with cell bodies of neurons." Neuropathool & Appl Neurobio 15, 531-542 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Allsop D, Haga S, Bruton C, Ishii T and Roberts GW: "Neurofibrillary tangles in some cases of dementia pugilistica share antigens with amyloid beta-protein of Alzheimer's disease." Amer J Pathol 136, 255-260 (1990).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi