• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1987 Fiscal Year Final Research Report Summary

Anti-bacterial defense mechanism of the urinary bladder.:Urinary substances which affect the bacterio-epitherial attachment.

Research Project

Project/Area Number 61440068
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

ORIKASA Seiichi  Prof. of Dept. Urol. Tohoku Univ. Sch. Med., 医学部・泌尿器科, 教授 (60001004)

Co-Investigator(Kenkyū-buntansha) YASUO Fukushi  Ass. of Dept. Urol. Tohoku Univ. Sch. Med., 医学部・泌尿器科, 助手 (50189932)
Project Period (FY) 1986 – 1987
KeywordsUrinary tract infection / Urinary mannose / Urinary fibronectin / Bacterial attachment / Pili of bacteria / 感染防御機構
Research Abstract

A quantitative observation of mannose in human urine was performed using high pressure liquid chromatography technique. In 24 of 137 cases, quantification was found to be possible from clear peaks of mannose. Except 3 cases, which were found to contain more than 60 g/ml of mannose, 21 cases showed to contain less than 20 g/ml of mannose. Furthermore, in 7strains of 11 mannose sensitive E.Coli strains, agglutination of goat red blood cells by E.Coli were prevented by the addition of less than 20 g/ml of mannose in the assay system.
We further observed the incidence of piliated bacteria cultured in human urine and two kinds of broth. This observation revealed that the incidence of piliated bacteria changes significantly according to not only the strains but also the state of culture, i.e., bacteria cultured in urine of patient who had frequent episodes of cystitis showed high incidence of piliation. Finally we tried to quantify the content of urinary fibronectine which is, in general, thought to be a factor of non-specific immunological mechanism and have a strong adhesiveness with Sta. Using sandwich assay with monoclonal antibodies, urine of healthy donor was found to contain 0.5<mu>g/ml on the average. Our preliminal observation revealed that preincubation of Sta. with human plasma fibronectine had some affect for prevention of bacterial attachment to bladder epithelial cell line HCV-29. Further observation about the small quantity substance in urine is in progress.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 鈴木 康義: 日本泌尿器科学会雑誌. 77. 574-580 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 折原 精一: 日本臨床. 44. 2544-2546 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木 康義: 日本泌尿器科学会雑誌. 78. 808-815 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 豊田 精一: 日本泌尿器科学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuyoshi Suzuki: "STUDIES OF ANTI-BACTERIAL DEFENSE MECHANISM OF URINARY BLADDER: 1)Activity of Phagocytosis of PMN in Urine 2) Viability, Cell Population and Activity of Phagocytosis of Urinary PMN" Jpn.J.Urol.77. 574-580 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiichi Orikasa: "Anti-bacterial mechanism in urinary tract" Nihon Rinsho. 44. 2544-2546 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuyoshi Suzuki: "THREE DIMENSIONAL OBSERVATION OF EARLY PHASE OF EXPERIMENT RAT CYSTITIS -Mainly of the activity of PMN in the lumen and on the surface-" Jpn. J. Urol.78. 808-815 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiichi Orikasa: "Anti-bacterial mecanism in urinary tract: Role of urinary mannose on the antibacterial mecanism" Jpn. J. Urol.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1989-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi