1987 Fiscal Year Final Research Report Summary
Developmental analysis of the role of call adhesion molecules in animal morphogenesis
| Project/Area Number |
61480021
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKEICHI Masatoshi Professor, Kyoto University, 理学部, 教授 (00025454)
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| Project Period (FY) |
1986 – 1987
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| Keywords | morphogenesis / cell adhesion molecules / cadherins / 細胞選別 |
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| Research Abstract |
I have isolated cDNAs encoding E-, N- and P-cadherin from cDNA libraries of mRNA derived from mouse and chick embryos. The analysis of the primary structure of these cadherin cDNAs showed that there are homologies in amino acid sequences among the three cadherins, suggesting that these molecules have a common ancester. I then attempted to introduce the cadherin cDNAs into L cells which have little cadherin activity. L cells transfected with the cDNAs synthesized cadherin polypeptides and acquired the Ca^<2+>-dependent aggregating activity. These cells were morphologically transformed from the fibroblast type to the epithelial type.
In order to examine the specificity of each cadherin molecule, I mixed L cells expressing E-cadherin with those expressing P-cadherin and allowed them to aggregate. The results showed that these cells aggregate independentely. Therefore, I concluded that each cadherin molecule has a specificity in cell-cell binding.
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