1988 Fiscal Year Final Research Report Summary

Grafting compatibility between plants of Orange subfamily and their use as citrus rootstocks.

Research Project

Project/Area Number	61480043
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	園芸・造園学
Research Institution	Saga University
Principal Investigator	IWAMASA Masao Professor, Faculty of Agriculture, Saga University, 農学部, 教授 (40039334)
Co-Investigator(Kenkyū-buntansha)	NITO Nobumasa Associate Professor, Faculty of Agriculture, Saga University, 農学部, 助教授 (80015809)
Project Period (FY)	1986 – 1988
Keywords	Orange subfamily plants / grafting compatibility / rootstock / micrografting / semi-micrografting / Microcitrus / Eremocitrus / Atalantia / Citropsis / Hesperethusa / Severinia / Glycosmis / Murraya / vitrification / Vitrification / 順化
Research Abstract	The grafting compatibility between plants of Orange subfamily were tested by micrografting and Semi-micrografting methods. semi-micrografting, the modification of micrografting to establish virus free plants in vitro, can be applied as an efficient short-term testing method for the grafting compatibility between plants of Orange subfamily. The juvenility of seedlings for rootstock affected the success of grafting, subsequent growth of scions and evaluation of compatibility. Seedlings grown under dark at 25゜C for 2 weeks were the best materials for this study. Poncirus, Fortunella, Microcitrus and Eremocitrus which are belonged to the true citrus fruit trees were easily grafted on rootstocks of Citrus spp. Citropsis and Atalantia in the near citrus fruit trees, and Hesperethusa and Pleiospermium in the primitive citrus fruit trees showed Compativility with citrus rootstocks. The compatibility between these genera re also shown by ordinary grafting. The reciprocal grafting between Murraya and Glycosmis which are belonged to the same subtribe were incompativle. Vitrified plants, anatomical and physiological disorder of in vitro grown plants, were acclimatized in the culture vessel using plugs with high aeration. Field experiment showed that Microcitrus, Atalantia and Severinia were promising as citrus rootstocks with cold tolerance.