1987 Fiscal Year Final Research Report Summary
Immuno-electrophysiologgy on ion channels in gastric secretory cells
| Project/Area Number |
61480096
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | TOYAMA MEDICAL AND PHAEMACEUTICAL UNIVERSITY |
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Principal Investigator |
TAKEGUCHI Horiaki Faculty of Phaemaceutical Sciences, Professor, 薬学部, 教授 (00019126)
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| Co-Investigator(Kenkyū-buntansha) |
ASANO Shinji Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (90167891)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Gastric secretion / Monoclonal antibody / Patch clamp / C1^- channel / H,K-ATPase |
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| Research Abstract |
Parietal cell seretes HC19 H,K-ATPase is an active H^+ pump in the apical membranes of the parietal cell. We found that tubulovesicles in the cell at the resting state C1^- channel in abbition to the H,K-ATPase and that the C1^- channel can be repoened by S-S cross-linking. We raised several monoclonal antibodies against tubulovesicles. One of them desiganted HK2032 partially inhibited H,K-ATPase and completely inhibited the C1^- channel. Present inhibition og both H,K-ATPade activity and the C1^- channel was direct evidence for that the C1^- channel was oart of the function of H,K-ATPase. Since Since Na,K-ATPase and Ca^<2+>-ATPase do not contain anion channel, the nature of H,K-ATPase is very specific. The C-terminal regions of the amino acid sequence of H,K-ATPase is very rich in cystein, which may engage in S-S<-->2SH (open<-->close) transformation of the C1 channel. The property of tubulovesicles in which C1^- channel was opened by cross-linking was the same as that of apical membrane vesicles in which C1^- channel was opened (without chemical modifications), indicationg that the fusion of tubulocesicles with the apical membrane occurs in the process of atimulation and it accompanies increase in C1^- conductance. An immunological study also showed that both H,K-ATPases in tubulovesicles and the apical membrane were wht same. %furthermore we obtained monoclonal antibodu HK4001 which completely inhibited H,K-ATPase activity and partially inhibited the C1^- conductance. Finally patch clamp study with monoclonal antibodies are in progress.
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Research Products
(14 results)
