1987 Fiscal Year Final Research Report Summary
Studies on the human glomerulonephritis inducing antigenic molecules using new nephritis models and monoclonal antibodies.
| Project/Area Number |
61480136
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Niigata University |
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Principal Investigator |
SHIMIZU Fujio Department of Immunology, Institute of Nephroiogy Niigata University School of Medicine Professor, 医学部, 教授 (40012728)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kazikiyo Department of Internal Medicine II, Niigata University School of Medicine Assist, 医学部, 助手 (70174919)
OITE Takashi Department of Immunology, Institute of Nephrology Niigata University School of M, 医学部, 助教授 (60018744)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Streptococcal cationic antigen / Monoclonal antibodies / Proteinuria / EHS nephropathy / heymann nephritis / Autoimmune membranous nephropathy / slit diaphragma |
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| Research Abstract |
Splenocytes from rats with membranous nephropathy which was induced by the immunization of EHS sarcoma or Fx1A, were fused with rat derived myeloma, Y-3 and some autoreactive monoclonal antibodies (MoAb) were produced. The single and mixed injections of MoAbs reactive with proximal tubular brush border or glomerular epithelial cell surface, lead to mesangial deposits formation demonstrated with immunofluorescence but not to any renal lisions.
Digestion of antigen with collagenase and intense and persistent immunization with the aids of Bordetella pertussis, these two new trials in immunization resulted in success in producing effectively many glomeruli binding MoAbs. One shot intravenous injection of the representative IgGl MoAb, 5-1-6, into rats induced massive proteinuria. Immunoelectron microscopy revealed that MoAb 5-1-6 binds in vitro to the surface of glomerular epithelial foot process, mainly to slit diaphragms. The molecular weight of antigenic molecules was about 51 Kd by immunoprecipitation. This MoAb provides a valuable tool for research into the mechanism of proteinuria.
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