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1987 Fiscal Year Final Research Report Summary

Studies on the human glomerulonephritis inducing antigenic molecules using new nephritis models and monoclonal antibodies.

Research Project

Project/Area Number 61480136
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNiigata University

Principal Investigator

SHIMIZU Fujio  Department of Immunology, Institute of Nephroiogy Niigata University School of Medicine Professor, 医学部, 教授 (40012728)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Kazikiyo  Department of Internal Medicine II, Niigata University School of Medicine Assist, 医学部, 助手 (70174919)
OITE Takashi  Department of Immunology, Institute of Nephrology Niigata University School of M, 医学部, 助教授 (60018744)
Project Period (FY) 1986 – 1987
KeywordsStreptococcal cationic antigen / Monoclonal antibodies / Proteinuria / EHS nephropathy / heymann nephritis / Autoimmune membranous nephropathy / slit diaphragma
Research Abstract

Splenocytes from rats with membranous nephropathy which was induced by the immunization of EHS sarcoma or Fx1A, were fused with rat derived myeloma, Y-3 and some autoreactive monoclonal antibodies (MoAb) were produced. The single and mixed injections of MoAbs reactive with proximal tubular brush border or glomerular epithelial cell surface, lead to mesangial deposits formation demonstrated with immunofluorescence but not to any renal lisions.
Digestion of antigen with collagenase and intense and persistent immunization with the aids of Bordetella pertussis, these two new trials in immunization resulted in success in producing effectively many glomeruli binding MoAbs. One shot intravenous injection of the representative IgGl MoAb, 5-1-6, into rats induced massive proteinuria. Immunoelectron microscopy revealed that MoAb 5-1-6 binds in vitro to the surface of glomerular epithelial foot process, mainly to slit diaphragms. The molecular weight of antigenic molecules was about 51 Kd by immunoprecipitation. This MoAb provides a valuable tool for research into the mechanism of proteinuria.

  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 中村享道: 新潟医学会雑誌. 100. 316-322 (1986)

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  • [Publications] Kimura, Satoshi: Clinical and Experimental Immunology. 64. 28-33 (1986)

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  • [Publications] 清水不二雄: Mebio. 3. 22-26 (1986)

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  • [Publications] 清水不二雄: Modern Urinalysis. 3. 22-28 (1987)

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  • [Publications] Kawachi, Hiroshi: Nephron. 47. 150-154 (1987)

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  • [Publications] 清水不二雄: Medical Practice. 5. 550-557 (1987)

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  • [Publications] 清水不二雄: 実験医学. 5. 1289-1294 (1987)

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  • [Publications] Nakamura, Takamichi: 412. (1988)

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  • [Publications] Morioka, Tetsuo: Nephron.

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  • [Publications] Kagami, Shoji: Clin. exp. Immunol.

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  • [Publications] Orikasa, Michiaki: J. Immunol.

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  • [Publications] Shimizu, Fujio 他edited by kano, k. et. al.: "Cellular, Moleclar and Genetic Approaches to Immunodeagnosis and Immunotherapy:Application of monoclonal, antibodies to immunpathological studies on renal diseases" University of Tokyo Press, 509(6) (1987)

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  • [Publications] 竹内正七編, 清水不二雄 他: "産婦人科Mook, 産婦人科医のための免疫学入門, 免疫学的手法の応用-組織学的応用" 金原出版, 362(10) (1987)

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  • [Publications] 越川昭三 他編 清水不二雄 他: "Annual Review腎臓1988(腎とモノクローナル抗体)" 中外医学社, 262(9) (1988)

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  • [Publications] 本田西男 他編 清水不二雄 他: "臨床腎臓病学(糸球体腎炎の病因-免疫学的発症・進展機序)" 朝倉書店, (1988)

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  • [Publications] Fujio,Shimizu: "Immunological factors involved in the induction and progression of nephritis." Mibio. 3. 22-26 (1986)

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  • [Publications] Satoshi,Kimura: "Monoclonal autoantibodies in Heymann nephritis." Clin. exp. Immunol.64. 28-33 (1986)

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  • [Publications] Takamichi,Nakamura: "Sclerotic lesions in the glomeruli of Buffalo/Mna rats." Nephron. 43. 50-55 (1986)

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  • [Publications] Takamichi,Nakamura: "Monoclonal antibodies to human renal glomerular components" Niigata Igakukai Zasshi. 100. 316-322 (1986)

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  • [Publications] Hiroshi,Kawachi: "Nephritogenicity of anti EHS sarcoma antibody." Nephron. 47. 150-154 (1987)

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  • [Publications] Fujio,Shimizu: "Kidney as a target of immunohistochemistry." Modern Urinalysis. 3. 22-28 (1987)

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  • [Publications] Fujio,Shimizu: "Mechanism of progression of Nephritis-- from the standpoint of immunolog" Medical Practice. 4. 550-557 (1987)

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  • [Publications] Fujio,Shimizu: "Clinical aspects of glomerulonephritis--What will be learned from basic medicine." Experimental Medicine. 5. 1289-1294 (1987)

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  • [Publications] Takamichi,Nakamura: "Monoclonal antibodies to human glomerular antigens." Virchows Archiv A Pathol. Anat. Histopathol.

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  • [Publications] Michiaki,Orikasa: "Massive proteinuria induced in rats by a single intravenous injection of a monoclonal antibody."

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  • [Publications] Fujio,Shimizu: Immunotherapy edited by K.Kano etal. University of Tokyo Press (Tokyo). Application of monoclonal antibodies to immunopathological studies on renal diseases. In Cellular, Molecular and Genetic Approaches to Immunodiagnosis and, 277-282 (1987)

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  • [Publications] Fujio,Shimizu: Chugai. Monoclonal antibodies applied to nephrology. In Annual Review Kidney 1988 edited by S.Koshikawa et al, 16-24 (1988)

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Published: 1989-03-30  

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