1987 Fiscal Year Final Research Report Summary
Studies of Rh antigen-related gene (RNA and DNA) and its Medico-Legal application
| Project/Area Number |
61480176
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Dept. of Legal Medicine, Shinshu University |
|---|
Principal Investigator |
FUKUSHIMA Hirofumi Shinshu University, 医学部, 助教授 (70135218)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Keywords | DNA analysis / mRNA / Rh antigen / LW antigen / RIA / ELISA |
|---|
| Research Abstract |
Enzyme-linked immumosorbent assay (ELISA) was used for ABO,Rh,fy,Jk,K Blood grouping. Positive and negatine reactions of anti-A, anti-B, anti-D, anti-C, anti-Fya, anti-Jka, and anti-K wells were ensily distinguished by this method. As pant of a study of frozm red cells, we tested the stability of the ABO and Rh antigens in frozem ulls. There were no significant changes of 125I-proteinA binding counts. There appear to be no barriers to the use of frozem uellss for fomily study or Rh blood type antigens. In order to isolate mRNA which is related to Rh 25(LW) antigen, we are now trying to purify total RNA from Japanese monky spleen. To obtain mRNA from exuss of other RNA, we are going to separate the RNA by chronatography on an oligo-(dT)-cellulose column.
|
