• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1987 Fiscal Year Final Research Report Summary

Detection and analysis of circulating immunecomplexes in the patients with connective tissue diseases by ELISA utilizing anti-C3d antibody.

Research Project

Project/Area Number 61480183
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionJuntendo University, School of Medicine

Principal Investigator

HIROSE SHUN-ICHI  Juntendo Univ.,School of Medicine.,Dept. of Med.,Prof. of Med., 医学部・内科, 教授 (70010147)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI OSAMU  Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 助手 (80163624)
HIRANO TAKAO  Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 講師 (10165186)
TAKASAKI YOSHINARI  Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 講師 (80154772)
Project Period (FY) 1986 – 1987
Keywordsautoimmune disease / connective tissue disease / Systemic lupus erythematosus Rheumatoid arthritis / circulating immunecomplexes / complement / 補佐 / C3dg
Research Abstract

Circulating immunecomplexes (CIC) have been detected among the patients with autoimmune diseases such as Systemic lupus erythematosus (SLE) and Rheumatoid arthritis(RA). In spite of the development of the assay system, etiology of organ involvemeent has not been fully characterized yet. Utilizing novel CIC detection assay with murine menoclonal anti-human C3dg antibody and monoclonal anti-human IgG Fc antibody (AC3dg), we havve tried to investigate the characterization of CIC and to study the effect of CIC for organ involvements. The assay (AC3dg) was sensitive and specific and has not been suffered by Rheumatoid factors in vivo and also in vitro. CIC level in each disease is as fllows; in SLE AC3dg 5.49+5.68ug/ml, Clg solidphase assay(ClqS) 3.74+4.00 ug/ml, in RA, AC3dg 5.56+4.51ug/ml,ClqS 2.43+1.96ug/ml, in mixed connective tissuee disease AC3dg 6.02+3.68ug/ml, ClqS 2.92+3.42ug/ml, in Progressive systemic sclerosis AC3dg 4.25+1.31ug/ml, ClqS 2.77+1.87ug/ml,in polyarteritis nodosa AC3dg 3.90+2.74ug/ml, ClqS 1.90+0.14ug/ml, and in Behchet's disease, AC3dg 4.98+2.26ug/ml, ClqS 2.38+1.72ug/ml. In RA patients, double filtration plasmapheresis decreased the CIC concentration from 6.01+3.62ug/ml down to 4.10+2.41ug/ml (decreace rate of 33%). And in SLE patients, low complementemia group showed higher CIC levels (AC3dg 7.62+11.81ug/ml, ClqS 4.17+2.91ug/ml) than normocomplementemia group (AC3dg 5.49+5.83ug/ml, ClqS 2.58+1.94ug/ml). On the other hand interestingly, patients with proteinuria showed lower CIC level(AC3dg 4.14+2.86ug/ml,ClqS 2.58+1.94ug/ml) than without proteinuria (AC3dg 5.76+6.09ug/ml, ClqS 3.84+4.24ug/ml). These data including the result in which AC3dg allows us to detect the antigen specific immunecomplexes formed in vitro such as CIC including PCNA (proliferating cell nuclear antigen), suggested that this may be te useful tool to study the etiology of the organ involvementand to follow up the patients.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 谷口修, 廣瀬俊一: 内科. 58. 753-756 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高崎芳成, 戸叫嘉明, 橋本博 他: 厚生省特定疾患混合性結合織病調査研究班昭和61年度報告書. 63-68 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI, O.;MIYAJIMA, M.;HIRANO, T. et al: J. of Clinical Immunol. 7. 441-448 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasukawa, R.;Tojo, T.;Takasaki, Y. et al: Excepta Medica ICS. 719. 41-47 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 廣瀬俊一: リウマチ. 27. 135-140

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIROSHI HASHIMOTO;HIROSHI TSUDA;SHUN-ICHI HIROSE, et al: J. of Rheumatology. 14. 497-501 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIRONO, T.;TANIGUHI, O.;KOGA, K. et al: "The appearance of Ia-T cells in systemic lupus etythematosus(in Cellular, Molecular and Genetic Approaches to immunodiagnosis and immunotherapy" University of Tokyo press, 441-445 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANIGUCHI,O., MIYAJIMA,M., HIRANO,T.,et al: "The Leu-1 B cell subpopulation in patient with Rheumatoid Arthtitis." J. of Clinical Immunol.7. 441-448 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KASUKAWA,R., TOJO,T., TAKASAKI,Y., et al: "Mixed connective tissue disease and anti-nuclear antibodies. Preliminary diagnosis criteria for classification of mixed connective tissue disease." Excepta Medica I C S. 719. 41-47 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HASHIMOTO,H., TSUDA,H., HIROSE,S., et al: "Differences in clinical and immunological findings of systemic lupus erythematosus related to age." J. of Rheumatology. 14. 497-5021 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANIGUCHI,O., HIROSE,S..: "Idiotype of anti-DNA antibody" Naika. 58. 753-756 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIROSE,S.: "Relation between immune complex and rheumatoid factor" Riumachi. 27. 135-140 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIRANO,T., TANIGUCHI,O., KOGA,K., et al: University of Tokyo press. The appearance of Ia-T cells in systemic lupus erythematosus. (in Cellular, molecular and genetic approaches to immunodiagnosis and immunotherapy.), 441-445 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1989-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi