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1987 Fiscal Year Final Research Report Summary

Immunological detection of specific protein from organs and blood of patients with Creutzfeldt-Jakob disease.

Research Project

Project/Area Number 61480202
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionKyushu University

Principal Investigator

TATEISHI Jun  Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (70033305)

Project Period (FY) 1986 – 1987
KeywordsCreutzfeldt-Jakob disease / Pathogen / Prion / Amyloid / クル班 / 脾臓
Research Abstract

Creutzfeldt-Jakob disease (CJD) is a intractable disease of central nervous system, causing transmissible dementia in human. The pathogen of CJD remains unknown, however, the prion hypothesis have been declared in scrapie infections.
I investigated the prion hypothesis in CJD. The purification protocols of prion yield amyloid proteins from CJD brains and non-transmissible amyloidosis. The amyloid fibril protein of CJD is composed of specific protein (prion protein), which is different from other amyloid proteins. Immunohistochemically, anti-prion protein reacted positively with kuru plaques in CJD brains, but not with other amyloid deposits. In addition, we reported that Congophilia of kuru plaque is modified by inactivation procedures on CJD agents. As the second step, we produced the antisera against human prion, mouse prion, and prion protein synthetic peptides. These antisera and a newly developed formic acid pretreatment revealed the very small kuru plaques in human and mouse CJD brain sections. In human CJD, almost all patients with long clinical couse (over 1 year) have kuru plaques in their brains. In mouse CJD, the kuru plaques are composed of mouse prion proteins, but not of human prion proteins. Therefore, kuru plaques can be one of the pathological hallmarks in CJD. The diagnosis of CJD is easily confirmed by the Western blotting technique using antiprion proteins. Prion protein can be detected in 5 mg (wet weight) of brain tissues, and 25 to 50 mg of spleen tissues from CJD-infected mice. In order to increase the sensitivity of the prion protein detection, we are going to establish the radioimmunoassay using our antisera.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Tateishi, J.: Lancet. ii. 1074 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi, J.: Lancet. ii. 1299-1300 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, T.: Acta Neuropathologica. 67. 272-278 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, T.: Bioscience Reports. 6. 459-465 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, T.: Annals of Neurology. 20. 204-208 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tashima, T.: Brain Research. 339. 80-86 (1986)

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  • [Publications] Tateishi, J.: Neuropathology. Suppl.3. 95-98 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi, J.: Lancet. i. 208 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, T.: Americal Journal of Pathology. 123. 407-412 (1986)

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  • [Publications] Kitamoto, T.: Laboratory Investigation. 57. 230-236 (1987)

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  • [Publications] Tateishi, J.: Annals of Neurology.

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      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, T.: American Journal of Pathology.

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      「研究成果報告書概要(和文)」より
  • [Publications] Mizutani T;Shiraki H Ed.;Tateishi, J.: "Clinicopathological aspects of Creutzfeldt-Jakob disease" Elsevier Science Publishers & Nishimura Co. Ltd., 325 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] Prushiner SB;McKinley, MP Ed.;Tateishi, J.: "Prions:Novel infectious pathogens causing scrapie and Creutzfeldt-Jakob disease." Acdemic Press, 540 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi,J.: "Transmission of Creutzfeldt-Jakob disease from human blood and urine into mice." lancet. ii. 1074 (1985)

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  • [Publications] Tateishi,J.: "Creutzfeldt-Jakob disease pathogen in growth hormone preparations is eliminatable." Lancet. ii. 1299-1300 (1985)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamoto,T.: "A new method to classify amyloid fibril proteins." Acta Neuropathologica. 67. 272-278 (1985)

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      「研究成果報告書概要(欧文)」より
  • [Publications] kitamoto,T.: "Scrapie-associated fibrils(SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis." Bioscience Reports. 6. 459-465 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamoto,T.: "Amyloid plaques in Creutzfeldt-Jakob disease stain with prion protein antibodies." Annals of Neurology. 20. 204-208 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashima,T.: "Congophilia in cerebral amyloidosis is modified by inactivation procedures on slow transmissible pathogens." Brain Research. 399. 80-86 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,J.: "Amyloid plaques in humans and animals with Creutzfeldt-Jakob disease Histological differentiation of amyloid." Neuropathology. suppl.3. 95-98 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,J.: "Membrane filtration of scrapie agent." Lancet. i. 208 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] kitamoto,T.: "Novel histochemical approaches to the prealbumin-related senile and familial forms of systemic amyloidosis." American Journal of Patholgy. 123. 407-412 (1986)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamoto,T.: "Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids." Laboratory Investigations. 57. 230-236 (1987)

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  • [Publications] tateishi,J.: "Gerstmann-Str ussler-Scheinker's disease: Immunohistological and experimental studies." Annals of Neurology.

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  • [Publications] Kitamoto,T.: "Immunohistochemical confirmation of Creutzfeldt-Jakob disease with long clinical course using amyloid plaque core antibodies." American Journal of Pathology.

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,J.: Elsevier Science Publishers & Nishimura Co. Ltd. edited. by Mizutani T, Shiraki H. Clinicopathological aspects of Creutzfeldt-Jakob disease, 325 (1985)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi,J.: Academic Press , edited by Prusiner S.B., Mckinley, M.P.Prions: Novel infectious parhogens causing scrapie and Creutzfeldt-Jakob disease., 540 (1987)

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      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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