1988 Fiscal Year Final Research Report Summary
Change of atrial natriuretic polypeptide receptors in the hypertensive state and the effect of drugs on these receptors.
| Project/Area Number |
61480209
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Okayama University, |
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Principal Investigator |
OTA Zensuke Third Dept of Int Med, Okayama Uni Med Sch, Prof., 医学部・第三内科, 教授 (90032870)
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| Co-Investigator(Kenkyū-buntansha) |
MAKINO Hirofumi Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部, 助手 (50165685)
HIRAKAWA Syuzo Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部附属病院, 助手 (60156651)
HASHIMOTO Kozo Third Dept of Int Med, Okayama Uni Med Sch, Lecturer, 医学部附属病院, 講師 (60033370)
OGAWA Norio Inst for Neurobiology, Okayama Uni Med Sch, Associate Prof., 医学部, 助教授 (90033208)
OGURA Toshio Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部, 助手 (80214097)
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| Project Period (FY) |
1986 – 1988
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| Keywords | atrial natriuretic polypeptide / hypertension / kidney receptor / autoradioqraphy / alpha-adrenerqic receptor / spontaneously hypertensive rat / DOCA salt hypertension |
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| Research Abstract |
To elucidate the characteristics of renal receptors for atrial natriuretic polypeptide(ANP) and their role in the regulation of blood pressure, we performed radiolabeled receptor assay(RRA) on rat renal membrane fractions and in vitro autoradiography (ARG) on frozen sections using 125I-labeled ANP. The density of 125I-labeled ANP in the kidneys of spontaneously hypertensive rats(SHR) was lower in all age groups(3-4, 5-7 and 12-15 weeks) than of control rats, suggesting that SHR have fewer binding sites genetically. A decrease in the number of ANP binding sites in 12 week-old SHR was observed following treatment with the anti-hypertensive diuretic indapamide. In DOCA salt hypertensive rats, plasma concentration of ANP was increased remarkably and the density of ANP receptors in the kidney was decreased. These result suggest that in this model ANP receptor activity was down regulated with increasing blood pressure. We also detected the ANP binding sites in the glomerulus of renal biopsy specimens, although a relationship between patients morbidity and the degree of ANP in the kidney was not determined. Differences of alpha adrenergic receptors in the kidneys of hypertensive and prehypertensive SHR were investigated and compared with those for ANP receptors. Both alpha1 and alpha2 adrenoceptors were more numerous in pre-hypertensive SHR as determined by computerized analysis of in vitro macro ARG of [3H -bunazosin and [3H -yohimbine.
We concluded that ANP play an important role in the control and progression of the hypertensive state.
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[Publications] Takatori,K.,Ogura,T.,Katayama,E.,Ota,Z.,Mizukawa,K.,Ogawa,N.: "Autoradiographic localization and computerized quantification of specific binding sites for alpha-1 and alpha2-adrenoceptors in spontaneously hypertensive rat kidney." The Journal of International Medical Research.
Description
「研究成果報告書概要(和文)」より
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[Publications] Katayama,E.,Ogura,T.,Takatori,K.,Mitsui,T.,Yamamoto,I.,Ota,Z.,Ogawa,N.,Mizukawa,K.: "Localization of α1-adrenoceptor in the rat kidney:in vitro autoradiography using [3H]-bunazosin." Research Communication in Chemical Pathology and Pharmacology. Vol.64. 205-213 (1989)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ogura,T.,Katayama,E.,Mitsui,T.,Takatori,T.,Ota,Z.,Mizukawa,K.,Ogawa,N.: "Properties of [3H]bunazosin binding in rat kidney." Clinical Therapeutics. Vol.10. 559-567 (1988)
Description
「研究成果報告書概要(和文)」より
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[Publications] Takatori,K., Ogura,T., Katayama,E., Ota,Z., Mizukawa,K. and Ogawa,N.: "Autoradiographic localization and computerized quantification of specific binding sites for alpha1- and alpha2-adrenoceptors in spontaneously hypertensive rat kidney." The Journal of International Medical Research.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Katayama,E. Ogura,T., Takatori,K., Mitsui,T., Yamamoto,I., Ota,Z., Ogawa,N. and Mizukawa,K.: "Localization of 1-adrenoceptor in the rat kidney: in vitro autoradiographyu sing[3H -bunazosin." Research Communications in Chemical Pathology and Pharmacology. Vol.64. 205-213 (1989)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ogura,T., Katayama,E., Mitsui,T., Takatori,K., Ota,Z., Mizukawa,K. and Ogawa,N.: "Properties of [3H bunazosin binding in rat kidney." Clinical Therapeutics. Vol.10. 559-567 (1988)
Description
「研究成果報告書概要(欧文)」より
