1988 Fiscal Year Final Research Report Summary
Studies of carcinogenesis of human papillomavirus
| Project/Area Number |
61480231
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | The Jikei University, School of Medicine |
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Principal Investigator |
NIIMURA Michihito Department of Dermatology The Jikei University School of Medicine, 皮膚科, 教授 (00010190)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Masakuni Department of Dermatology The Jikei University School of Medicine, 皮膚科, 助手 (80169292)
ISHIDA Takashi Department of Dermatology The Jikei University School of Medicine, 皮膚科, 助手 (90159731)
TOZAWA Takayuki Department of Dermatology The Jikei University School of Medicine, 皮膚科, 講師 (50155529)
HONDA Mariko Department of Dermatology The Jikei University School of Medicine, 皮膚科, 講師 (20100919)
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| Project Period (FY) |
1986 – 1988
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| Keywords | human papillomavirus / viral wart / viral carcinogenesis / Bowen's disease / condyloma acuminatum |
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| Research Abstract |
The human papillomavirus (HPV) inmduce warts in skin and mucosa. Wart can take various types of clinical pictures including common warts, flat warts, condyloma acuminata tnd laryngeal papillomas. there are even warts that undergo malignant transformation, as in epidermodysplasia verruciformis and genital Bowen's disease. We have been to elucicidate viral DNA base pair sequences and have detected 56 different types of HPV. The viral type and clinical pictures are generally related. In patients who have congyloma acuminata, are usually caused by HPV-6 or 11. If one take cervical carcinoma and examine for them for HPV-DNA, 16 or 18 DNA can be detected in high percentage. Cervical cancer bears a close relationship to HPV-DNA. Using biotin-labeled HPV-DNA probes, the in situ hybridization were used to detect HPV-DNA in paraffin embedded sections of specimens from 64 male and female patients with condyloma acuminata. 58 were positive for HPV-DNA : 39 for HPV-6, 14 for HPV-11, 2 for HPV-16, 1 for HPV-2 and 2 for unclassified HPV types. Thus 83 % of anogenital warts contained HPV-6 or 11 sequences. Surprisingly, HPV-16 DNA which usually detected from malignant lesions was detected in the anal lesions of two male patients. Histologically the lesion showed papillomatosis and coilocytosis with atypia, and HPV capsid antigen was detected in an anal lesion. On epidermodysplasia verruciformis, we have established a cell line from an intraperitoneal metasiasis of skin cancer of a patient with epidermodysplasia verruciformis. HPV-5 DNAs were detected in these cultured cells. Also we have cloned HPV 5 DNA from the patient and clarified 7764 base pair sequences.
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