1987 Fiscal Year Final Research Report Summary

Adjuvant therapy for lung cancer. - a missile therapy using monoclonal antibody

Research Project

Project/Area Number	61480296
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Thoracic surgery
Research Institution	Mie University
Principal Investigator	NAMIKAWA Shoji Mie University Hospital, Lecturer, 医学部附属病院, 講師 (50024716)
Project Period (FY)	1986 – 1987
Keywords	Monoclonal antibody associated lung cancer / Missile therapy / 2type glucose conjugated antigen / FH-6
Research Abstract	The so called missile therapy in order to achivev the perfect adjuvant therapy against operated lung cancer were intended, and the following works ere made. 1) Preparation os highly effective monoclonal antibody (MOAB) associated with lung cancer. 2) Investigation of the mutual reactions with the cases of human lung cancer tissues and other neoplasms, and simultaneously of the incorporation of MOAB into normal organs by immunohistochemical staining, thus pursuing its localization. 3) Preparation of nude mice on which human lung cancer was transplanted, and MOAB conjugated with I^<125> was infused into the animals, anmd the incorporation into the trrget or localization in other organs were examined with autoradiography. 4) Decision of effective MOAB and conjugation with anti-cancer drugs were made to investigate the effect. It was found as a result that the MOAB established by us was useful for pathological adiagnosis, but it was found inappropriate for missile therapy for some reasons. Subsequently, we experimented a similar reaction with a MOAB reacting Le^x-i antigen, which is type 2 glucose-cojugated antigen and it was found as a result that it showed a specifically strong reactivity to adenocarcinoma of the lung, and its crossreaction with the normal lung tisssue was shown only in the bronchial gland. Therefore, we infused this MOAB conjugated with I^<125> into already prepared nude mice, and their sections was made its usefulness was found out, and now the ADR liposome with MOAB is made.