1987 Fiscal Year Final Research Report Summary

Exterimental study on heart lung transplantation in monkey

Research Project

Project/Area Number	61480298
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Thoracic surgery
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	OGA Koichi Kyoto Prefectural University of Medicine, 医学部, 助教授 (40079843)
Co-Investigator(Kenkyū-buntansha)	KANKI Yutaka Kyoto Prefectural University of Medicine, 医学部, 助手 (40169785) WADA Yukio Kyoto Prefectural University of Medicine, 医学部, 助手 (90167213) SHIRAKATA Shuji Kyoto Prefectural University of Medicine, 医学部, 講師 (80117887)
Project Period (FY)	1986 – 1987
Keywords	Heart-lung transplantation / Japanese monkey / Histochemistry / モノクロナル抗体
Research Abstract	Orthotopic heart-lung transplantation is performed using Japanese monkeys. Bleeding was the first trouble, and weaning from extracorporeal circulation was difficult. By technical improvement including the proper selection of sutures, and using of another monkey as a blood donor, hemostasis was achieved, and weaning from ECC became easy. Then the next trouble was the deterioration of lung. Atelectasis, edema, bleeding developed from just after recirculation, and resulted in the low PaO_2, elevation of inspiratory pressure of respirator. All cases died within 6 hours after weaning of ECC. Care was made not to touch the lungs as possible during resection and transplantation of heart and lungs, and Ep3 solution which was reported by Tohoku University was used to protect the lungs from injury caused by ischemia. Ice slush was also used to cool the lungs during transplantation. These procedures appearently improved the lungs condition of the heart and lungs after ECC, 44 hours was the longest survival time. Investigation of the early detection of rejection and the morphological aspects of nerve fibers in the transplanted, denervated heart and lungs were made by next experiments. Left hemi-lung was transplanted orthotopically, and the heart was implanted in the abdomen. Five days after transplantation, nerve fibers in the transplanted bronchus and atrio-ventricular node of the heart showed almost normal Ch-E activity. Investigation to detect the rejection was made by histochemistry and using the monoclonal antibody.