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1987 Fiscal Year Final Research Report Summary

Multiple analysis of brain metabolism in ischemia

Research Project

Project/Area Number 61480304
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YOSHIMOTO Takashi  Tokoku University School of Medicine (Associate Professor), 医学部(脳神経外科), 助教授 (50091765)

Co-Investigator(Kenkyū-buntansha) KAMEYAMA Motonobu  Tohoku University School of Medicine, 医学部(脳神経外科), 助手 (70169647)
MIZOI Kazuo  Tohoku University School of Medicine (KAWAZOE,Yosh), 医学部(脳神経外科), 助手 (70157519)
IDO Tatsuo  Cyric, Tohoku University (Professor), サンクロトロンラジオアイソトープセンター(RI製造), 教授 (80134063)
Project Period (FY) 1986 – 1987
KeywordsComplete forebrain ischemia / reperfusion / cerebral blod flow / glucose metabolism / amino acid metabolism / No-reflow phenomenone / Mannitol / マンニットール
Research Abstract

An experimental model of rat forebrain ischemia regulated with perfusion method was created. The preparation mainly consists of the occlusion of bilateral externel earotid, pterygopalatine arteries and basilar artery, then the brain is perfused by the femoral artery blood using a pump through bilateral common carotid arteries. EEG findings and qualitative CBF by 14C-iodoantipyrine autoradiography of this model were studied, which indicate the possible usefulness of this model in the basic investigation of cerebral ischemia.
A seven vessel occlusion model in the rat (occlusion of bilateral pterygopalatine, external carotid, common carotid arteries, and basilar artery) that produced complete forebrain ischemia without systemic hypotension was developted and used for following studies.
After 5min reperfusion following 30min ischemia, impaired reperfusion pattern was verified mainly in basal ganglia by CBF autoradiography, while, the recirculation pattern in mannitol pretreated animals was homogeneous and hyperemic. This indicates that mannitol prevented impairment of reperfusion after temporary ischemia.
Glucose and amino acid metabolism in reperfusion phase after ischemia was studied simultaneously by 18FDG and 14C-leucine multiple labeled autoradiography. The dissociation between blucose and amino acid metabolism was observed in deep part of the brain where the reperfusion tended to be impaired.
These results will provide us an important clue for undestanding pathophisiological corellation between metabolism and postischemic damage of the brain in reperfusion.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 白根礼造: 東北脳血管障害懇談会誌. 8. 157-162 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reizo SHIRANE: Journal of Neurosurgery.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reizo SHIRANE: Journal of Newosurgery.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reizo,SHIRANE: "A new modcl of cerebral ischemia in the rat regulatid with peyusion method" Cerebrovascular Diseace Tohoku. 8. 157-162 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reizo,SHIRANE: "Completc forebrain ischcmia in the rat" Journal of Nemosurgey.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rizo,SHIRANE: "Effect of prctreatment with snaunitol on poslischemic reperyusion after transient glohal ischemia in rats" Journal of Newosurgey.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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