1988 Fiscal Year Final Research Report Summary
Human trabecular meshwork organ culture.
| Project/Area Number |
61480365
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Shinshu University |
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Principal Investigator |
SEGAWA Katsuzo Department of Ophthalmology. Shinshu University School of Medicine, 医学眼科学教室, 教授 (70020857)
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| Co-Investigator(Kenkyū-buntansha) |
HOYA Takuo Department of Ophthalmology. Shinshu University School of Medicine, 医学部附属病院眼科, 助手 (00201757)
MIYAZAKI Morito Department of Ophthalmology. Shinshu University School of Medicine, 医学部附属病院眼科, 助手 (90174169)
URAKAWA Yoshio Department of Ophthalmology. Shinshu University School of Medicine, 医学眼科学教室, 助手 (10160334)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Trabecular meshwork / Culture / Transmission electron microscopy / Scanning electron microscopy / Human eye / 房水流出路 |
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| Research Abstract |
Human trabecular meshwork obtained from postmortem eyes was maintained in organ culture condition up to 12 weeks using solid agar method.
The effects of various agents upon human trabecular meshwork were studied using organ culture method as mentioned above. Various agents were as follows: (1) gold, (2) iron, (3) viscoelastic materials (methylcellulose (MC), sodium hyaluronate (SH) and chondroitin-6-sulfate (CDS)), (4) anti-glaucoma drugs (pilocarpine, epinephrine, -blockers (timolol, carteolol, befunolol)), (5) vitamin a, (6) vitamin C, (7) steroid (dexamethasone), and (8) photocoagulation.
Gold, iron, SH and CDS activated the trabecular cells to cause deposition of extracellular material. MC caused little change in trabecular meshwork cells and extracellular material.
Pilocarpine induced swelling of mitochondria, while epinephrine induced rounded cells and separation of cells from trabecular sheets. Beta-blockers induced accumulation of lysosome in cytoplasm.
A decrease of extracellular material and an increase of empty space occurred one day after addition of vitamin A, while an increase of extracellular material and a decrease of empty space one or two weeks after addition of vitamin C in the endothelial meshwork.
In a few cases an increase of extracellular material was observed, and in others no increase of extracellular material was observed two weeks after addition of dexamethasone. These results indicated that the former were steroid responders.
Photocoagulation on trabecular meshwork induced tissue defect and cell loss, and increased empty space in the endothelial meshwork.
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