1987 Fiscal Year Final Research Report Summary
Tetracycline-labelling in decalcified bone section
| Project/Area Number |
61480375
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YOSHIKI Shusaku Professor, School of Dentistry, Showa University, 歯学部, 教授 (30085740)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Yuichi Assistant, School of Dentistry, Showa University, 歯学部, 助手 (20180408)
YAMAZAKI Tohru Lecturer, School of Dentistry, Showa University, 歯学部, 講師 (40112734)
TACHIKAWA Tetsuhiko Assist. Prof., School of Dentistry, Showa University, 歯学部, 助教授 (10085772)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Hard tissues / Decalcified sections / TC標識 |
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| Research Abstract |
Evidence of tetracycline(TC)-labelling in decalcified dentin was reported and TC uptake by a part of the organic component in calcified tissues was reconfirmed ( Simpson, 1981). This report deals with an experimental study to know possible demonstration of TC-labelling in decalcifed bone section. The results were as follows: (1) About 5 week-immersion fixation of bone tissues by 10% neutral formalin was necessary for possile demonstration of TC-labelling in decalcifed bone sections. (2) Additional fixation by cyanuric chloride after formalin fixation showed little effect for the preservation of TC-labelling in the sections. (3) Decalcification by neutral EDTA must be applied for the same purpose. (4) Degree of TC-labelling demonstrated in decalcified bone sections was varied with a type of administrated tetracycline.
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