1987 Fiscal Year Final Research Report Summary
The role of genes associated with cell growth in proliferating diseases
| Project/Area Number |
61480434
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般
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| Research Institution | Hokkaido University. |
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Principal Investigator |
KUZUMAKI Noboru Hokkaido University School of Medicine, 医学部, 教授 (80091445)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Psoriasis / oncogenes / oncogene products / monoclonal antibody / mRNA |
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| Research Abstract |
Expressions of several protooncogenes related with cell growth were examined in psoriatic tissues comparing those in normal skin tissues using monoclonal antibody and Northern blotting techuiques. Immunofluorescence studies using monoclonal antibody rp35 reacted with ras p21 revealed that only based layers of epidermis were stained in normal skin, while whole layers of epidermis were stained in psoriatic tissues, sugges ting overproduction of ras p21 in this disease. Northern blotting techniques with poly a(+) mRNA derived from psoriatic tissues and normal skin demonstrated that levels of fos and myc transcripts were decreased, while that of K-ras transcript was increased in psoriatic tissues in comparison with those in normal skins. All these data suggest association of protooncogenes with pathogenesis of psoriasis.
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