1988 Fiscal Year Final Research Report Summary
Analysis of the genes in lymphocyte differentiation.
| Project/Area Number |
61480478
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | FUJITA-GAKUEN HEALTH UNIVERSITY |
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Principal Investigator |
KUROSAWA Yoshikazu Institute for Comprehensive Medical Science, Fujita-Gakuen Health university, 総合医科学研究所・医学部, 教授 (10109259)
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| Project Period (FY) |
1986 – 1988
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| Keywords | immunoglobulin gene / IgM- and IgG- double bearing cell / Suncus murinus / ジャコウネズミ / ヒトIgD産生株 / トランススプライシング |
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| Research Abstract |
We are analyzing functions of sigma structure in human immunoglobulin heavy chain gene loci: _ located downstream of enhancer, _ and _ located between and genes, _ located upstream of s_ . In this year, we carried out the following experiments.
1)We isolated immunoglobulin gene from DNA of the insectivors suncus murinus, and determined its nucleotide sequence. Comparison of nucleotide sequences between man mouse and suncus will give us information for biologically meaningful regions.
2. We established a B cell line expressing both IgM and IgG on the cell surface by transformation of peripheral plood cells of a patient with hyper IgM immunodeficiency using epstein-barr viruses. The data showed that in double isotype-bearing cells, one VHDHJH exon in this transript is alternatively spliced to C_ or C_ without DNA rearrangement. The defect in this disease could be at the S-S recombination stage.
3) Although the frequency of IgD-secreting cells is extremely low in mouse, about 1% of the patients with myeloma produce IgD in humam. We analyzed dna from human IgD myelomas. The data indicated that homologous recombination between _ and _ sequences mediates class switch from to in human, and that it occurs via unequal crossing over between sister chromatids or daughter chromosomes.
4)We are analyzing mechanism alternative rna splicing in ^- and ^- double producers.
5)We are analyzing precence of discontinuous transcription followed by trans RNA splicing.
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