| Research Abstract |
Construction and Examination of Animal Models of Cerebrovascular Disorders. Using such animal models of cerebrovascular disorders as (1) rats in which the carotid arteries were ligated either bilaterally or unilaterally(ligation animals), (2) rats in which only the vertebral artery was escharotized or after escharotization of the vertebral artery, the animals were either bilaterally or unilaterally ligated (escharotized animals) (3) rats in which beads with a diameter of 3 m were infused through the carotid artery(beads animals), during the last fiscal year we reported on the effects of administering various cerebral circulatory dynamics enhanicng drugs and cerebral metabolism activators on intracerebal catecholamine and GABA. During this fiscal year we wrer able to obtain uniform results by conducting highly sensitive measurements of Choline(CH) and Acetylcholine(ACH) based on pyrolysis, as chromatography and mass spectrometry(PY/GC/MS). During this research, as our pyrolysis equipmen
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t we used an induced heat furnace and for gas chromatography we used a capillary column and thereby studied a method of analysis which is simple, highly sensitive and has good reproducibility. That is to say, in both the packed column and capillary column we were able to obtain sharp chromats of the 4 components, Ach, Ch, Propionyl choline(Pch) and butyryl choline(Bch) and compared with the packed column, we were able to obtain a high performance of 164 X in number of theoretical plate and 6.95 X in separation capacity. We were able to obtain 4 kinds of representative fragments of Ach, namely, m/z 58, m/z 71, m/z 87 and m/z 131, however, in order not to obtain particular molecular ions by the EI method, we attempted a selective method of analysis by the CI method. Also, since the quantitation of Ch was poor, we combined Ch with Pch and made determinations and found that we obtained the same results as with standard pch. there is a particular mass spectrum for each component and each of the 4 components display a standard peak of the pseudo-molecular peak (MH^+) so that we were able to obtain peaks with few fragment inos (Ch: m/z 90, Ach: m/z 132, Pch: m/z 146 and Bch: m/z 160. From the above, it can be seen that based on the PY/ GC/MS method using cadillary columns. We were able to obtain research results that were simple, highly sensitive, and had good reproducibility. Less
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