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1987 Fiscal Year Final Research Report Summary

Cytoplasmic control of the cell cycle

Research Project

Project/Area Number 61490016
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionKyoto University

Principal Investigator

YONEDA Mitsuki  Faculty of Science, Kyoto University, 理学部, 教授 (50017183)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Yoshihiko K.  Faculty of Science, Kyoto University, 理学部, 助手 (60135514)
KUBOTA Hirochi Y.  Faculty of Science, Kyoto University, 理学部, 助手 (40115837)
Project Period (FY) 1986 – 1987
KeywordsXenopus / Calcium / Fertilization / Activation / Cell cycle / Sea urchin / Androgenesis / 付活
Research Abstract

(1) Mechanical properties of sea-urchin eggs as an index of the cell cycle. Profile of the spherical shells flattened under two parallel plates was calculated to obtain the relation between the compressing force and the degree of flattening. Matching it with observed relation of force-deformation in sea-urchin eggs, we found Young's modulus of the egg sufrace of 10^2 dyne/cm^2, being 1/10 of an estimate made by Hiramoto.
(2) Wave of free calcium on activation of Xenopus eggs. Unfertilized eggs injected with aequorin were activated by pricking and observed with a two-dimensional photon-counting system tith an image processor. A free Ca incredse was initiated around the point of pricking, and this state of high Ca propagated as a wave, similar to the wave of change in brightness of the surface on activation, knwn as "activation wave". By correlating images of aequorin luminescence and the brightness of the surface, we found temporal coincidence between the Ca wave and the activation wave.
(3) Correlation of the Calcium wave and the ultrastructure of the cortex in fertilized Xenopus eggs. Difficulty in finding Ca wave in fertilized frog eggs due to loss of their fertility on treating them with Ca-fiee madium (to prevent egg activation during injection of aequorin) was overcome by injecting aequorin into coelomic oocytes, and by placing them into the coelom of an albino-type female. Wild-type eggs found among albino eggs from the albino mother was then fertilized by sperm. We found a Ca wave in fertilized eggs. The Ca wave coincided with the relaxation zone of the activation wave, where exocytosis of cortical granules and elongation of microvilli were taking place.
(4) Developmental timing in androgenesis. Careful observation of the development of the pairs of embryos derived from nucleate and non-nucleate fragments of single unfertilized eggs indicated that the delay is entirely dueto extension of sperm amphiaster stage.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Miramoto, Y. & Yoneda, M: Methods im Cell Biology. 27. 443-456 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubota, H. Y. ;Yoshimoto, Y. ;Yoneda, M. & Hiromoto, Y.: Developmental Biology. 119. 129-136 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maruyama, Y. K.: The Journal of Experimental Zoology. 238. 241-248 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maruyama, Y. K. ;Yamamoto, K. & Mita-Miyazawa, I.: Methods in Cell Biology. 27. 325-344 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masho, R. & Kubota, H. Y.: Development, Growth & Differentiltion. 28. 113-123 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoneda, M.: Methods in Cell Biology. 27. 421-434 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoneda, M.: Zoological Science. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiramoto,Y.and Yoneda,M.: "Determination of mechanical properties of the egg by the sessile drop method." Mathods in Cell Biology. 27. 443-456 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubota,H.Y.,Yoshimoto,Y.,Yoneda,M. and Hiramoto,Y.: "Free calcium wave upon activation in Xenopus egg." Developmental Biology. 119. 129-136 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruyama,Y.K.: "Induction of sea cucumber oocyte maturation by starfish radial nerve extiacts." The Journal of Experimental Zoology. 238. 241-248 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruyama,Y.K.,Yamamoto,K. and Mita-Miyazawa,I.: "Manipulative methods for analyzing embryogenesis." Methods in Cell Biology. 27. 325-344 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masho,R. and Kubota,H.Y.: "Developmental fates of blastomeres of eight-cell-stage Xenopus laevis embryos." Development, Growth & Differntiation. 28. 113-123 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoneda,M.: "The compression method for determining the surface force." Methods in cell Biology. 27. 421-434 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoneda,M.: "Computed profiles of compressed sea-urchin eggs with elastic membrane." Zoological Science. (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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