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1987 Fiscal Year Final Research Report Summary

A Ca-binding protein involved in the cell adhesion of sea urchin embryo.

Research Project

Project/Area Number 61540512
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionSAITAMA UNIVERSITY

Principal Investigator

TONEGAWA Yasuto  Fac. of Science, Associate professor, 理学部, 助教授 (70008837)

Project Period (FY) 1986 – 1987
KeywordsSea urchin embryo / Cell adhesion molecule / Ca-binding protein / Lectin / Glycoprotein / レクチン
Research Abstract

A cell-aggregating activity was extraced as a gigantic sugar-protein complex from the supernatant of cell dissociation of a sea urchin embryo, Hemicentrotus pulcherrimus. A Ca-binding protrein was isolated from the complex by DEAE-cellulose and purified. It was an acidic glycoprotein(pI 3) around 1,000K. It bound ^<45>Ca pH dependently, maximal at the pH of sea water(pH 8) and the dissociation constant was 4x10^<-4> M. From amino acid analysis, it was shown to be a gla-protein containing 0.5% of -carboxyglutamic acid. Inhibitors of gla-protein were shown to disturb cell adhesion in vitro and in situ.
On the other hand, the binding of this Ca-binding protein to the cell surface was shown to be mediated by another mechanism. The embryos of H. pulcherrimus was shown to be dissociated species-specifically by the wheat germ agglutinin(WGA). And the cell reaggregation by this protein was inhibited by GlcNAc. Also, the Ca-binding protein competed with WGA in hemagglutination.
WGA receptor was extracted by the aid of detergent from dissociated cells and purified. It reacted with the Ca-binding protein and disturbed cell adhesion in situ. Accordingly, it is assumed to be the cell surface receptor for the Ca-binding protein. In conclusion, the cell adhesion molecule of sea urchin embryo was shown to be a bifunctional glycoprotein containing Ca-binding and sugar-binding domains.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Y.Tonegawa;T.Doi;T.Suzuki: Prog.Clin.Biol.Res.217B. 211-214 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Numata;Y.Toneagwa: Zool.Sci.3. 1059 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tonegawa;M.Tanaka;H.Tsukiura;Y.Oonuki: Proc.9th.Int.Symp.Glycoconjugates. G65 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tonegawa;N.Horie;I.Itabashi;H.Isowa;K.Abe;M.Watanabe: Proc.4th Europ.Carbohydrate Symp.B19 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kinoshita;K.Yoshii;Y.Tonagawa: Exptl.Cell Res.(1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tonegawa, T.Doi and T.Suzuki: "A Ca-binding protein and an endogenous lectin from sea urchin embryos." Proc.Clin.Biol.Res.217B. 211-214 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Numata and Y.Tonegawa: "Isolation of a receptor glycopeptide for the endogenous lectin of sea urchin embryo." Zool. Sci.3. 1059 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Tonegawa, M.Tanaka, H.Tsukiura and Y.Oonuki: "Localization of a hyaluronidase-sensitive glycan in the nucleus of undifferentiated embryos." Proc. 9th Int. Symp. Glycoconjugates. g 65 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Tonegawa, N.Horie, I.Itabashi, H.Isowa, K.Abe and M.Watanabe: "Sugar-recognizing system is involoved in the species-specific cell adhesion of sea urchin embryos." Proc. 4th Europ. Carbohydrate Symp.B 19 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kinoshita, K.Yoshii and Y.Tonegawa: "Specific binding of lectins with the nucleus of the sea urchin embryo and changes in the lectin affinity of the embryonic chromatin during the course of development." Exptl. Cell Res.(1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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