1987 Fiscal Year Final Research Report Summary
DEVELOPMENTAL GENETICS IN TESTICULAR TERATOCARCINOGENESIS AND GERM CELL DEFICIENCY CAUSED BY THE GENE ter IN MICE.
| Project/Area Number |
61540516
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | SHIZUOKA UNIVERSITY |
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Principal Investigator |
NOGUCHI Motoko BIOLOGICAL INSTITUTE, FACULTY OF SCIENCE, SHIZUOKA UNIVERSITY, 理学部, 助教授 (40021951)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Testcular teratomas / Teratocarcinogenesis / Primordial germ cells / Germ cell deficiency / Recessive mutant gene / teratoma(ter) / 劣性突然変異遺伝子teratoma(ter) / terコンジェニック系統 |
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| Research Abstract |
TESTICULAR TERATOMAS IN MICE ARE CONGENITAL ABNORMALITIES ORIGINATING WITHIN SEMINIFEROUS TUBULES IN FETAL TESTES FROM SINGLE PRIMORDIAL GERM CELLS(PGCS). RECENTLY NOGUCHI AND NOGUCHI FOUND THAT THE SINGLE RECESSIVE MUTANT GENE ter CAUSES GERM CELL DEFICIENCY, ACCOMPANIED BY A HIGH INCIDENCE OF TESTICULAR TERATOMAS IN ter HOMOZYGOUS MICE IN 129/Sv-ter STRAIN (JNCI,75,385,1985). IN THIS RESEARCH,THE EXPRESSION OF THE GENE ter IN GERM CELL DEFICIENCY AND TESTICULAR TERATOCARCINOGENESIS WAS ANALYSED FROM THE VIEWPOINT OF DEVELOPMENTAL GENETICS.
1. IN ORDER TO ESTABLISH THE DEVELOPMENTAL BASIS OF STERILITY AND TESTICULAR TERATOCARCINOGENESIS IN ter/ter MICE, THE NUMBER, LOCATION AND MORPHOLOGY OF PGCS WERE EXAMINED IN 8.5-12.5 DAYS EMBRYOS FROM MATING OF +/ter x +/ter AND+/+ x +/+. IT WAS SHOWN THAT THE GENE ter CAUSES EXTRA-GONADAL PGCS DEFICIENCY,NOT ACCOMPANIED BY EXTRACONADAL TERATOCARCINOGENESIS IN ter HOMOZYGOTES IN9.5-12.5 DAYS EMBRYOS IN 129/Sv-ter MICE.
2. IN ORDER TO EXAMINE THE INFLUENCE OF A GENETIC BACKGROUND ON THE ter ACTION, THE GENE ter WAS INTRODUCED ONTO C57BL/6J GENETIC BACKGROUND BY BACKCOSSING FOR 9 GENERATIONS. IT WAS CONCLUDED THAT THE GENE ter ON C57BL/6J GENETIC BACKGROUND IN ter CONGENIC MICE CAUSES GERM CELL DEFICIENCY IN ter HOMOZYGOTES AND THAT GENE(S) OTHER THAN ter IN 129/Sv ARE INVOLVED IN DETERMINING SUSCEPTIBILITY TO TESTICULAR TERATOCARCINOGENESIS IN PGCS.
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