1987 Fiscal Year Final Research Report Summary
Regulation of the differentiation of multipotent and erythroid stem cells
| Project/Area Number |
61540538
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
MORIOKA Kiyokazu The Tokyo Metropolitan Institute of Medical Science,Honkomagome 3-18-22,Bunkuo-ku,Tokyo 113,Japan, 腫瘍生化学研究部門, 主任研究員 (10124497)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Kiyoko The Tokyo Metropolitan Institute of Medical Science,Honkomagome 3-18-22,Bunkyo-k, 腫瘍生化学研究部門, 研究員 (40124474)
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| Project Period (FY) |
1986 – 1987
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| Keywords | conditioned medium / inhibitor of erythropoiesis / CFU-E / BFU-E / hemoglobin / bone marrow / spleen |
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| Research Abstract |
Pokeweed mitogen-stimulated spleen conditioned medium and bone marrw conditioned medium were shown to contain substance(S) that was able to suppress colony formation by early (BFU-E) and late (CFU-E) erythroid progenitorors which had been induced by the addition of erythropoietin to a semi-solid culture system of syrian hamster bone marrow cells. Incubation of the conditioned medium at 56゜C,30 min resulted in an almost complete loss of the suppessing activity. Dialysis and gel-filtration experiments suggest that the activity is mainly due to high molecular weight substance(S). The activity in the bone marrow conditined medium was destroyed by a trypsin-treatment,while in the spleen conditioned medium was partially active after the same treatment.
Homeglobin synthesis of yolk-sac or adult bone marrow erythroid cells was significantly enhanced by the presence of adult bone morrow non-erythroid cells or thir conditined medium. Pokeweed mitogen-stimulated spleen conditined medium was also effective. Details are under investigation.
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