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1987 Fiscal Year Final Research Report Summary

Fabrication of Semiconductor Sensor for Immunological Measurement

Research Project

Project/Area Number 61550292
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 計測・制御工学
Research InstitutionUniversity of Tokyo

Principal Investigator

IKEDA Kenji  Faculty of Medicine, University of Tokyo, 医学部, 助手 (70010030)

Co-Investigator(Kenkyū-buntansha) WATANABE Akira  Faculty of Medicine, University of Tokyo, 医学部, 助教授 (00009937)
SAITO Masao  Faculty of Medicine, University of Tokyo, 医学部, 教授 (60010708)
Project Period (FY) 1986 – 1987
KeywordsImmunological Measurement / Semiconductor Sensor / Thin Organic Film / Plasma-polymerization / 固定化タンパク質
Research Abstract

Fabrication process of semiconductor sensors for immunological measurement was investigated. The device fabrication-process utilized in this work consists of two steps; (1) deposition of thin organic film by plasma technique, and (2) protein immobilization. It is desirable that the electrical properties of organic film are stable and that high density of protein molecules can be immobilized in an orientation-controlled manner.
Electrical property of the organic film was studied using metal/insulator/semiconductor structured capacitors. Plasma-polymerized organic film was deposited onto SiO_2/Si structured substrates. After depositing the Al electrodes, capasitance-voltage characteristics were measured. The hysteresis and the shift of flatband voltage were observed. As far as the technological application to semiconductor sensors is concerned, the above two properties are disadvantageous features. Thus, attempts were made to reduce those effects by heat treatment. The effect was significant. The hysteresis width as well as the shift of flatband voltage were reduced. The best result was obtained in the case of the heat treatment at 200 ゜C for 30 min. in N_2 atmosphere.
Immobilization reaction was performed as the following steps; first, amino-groups were introduced to the surface of the plasma-polymerized film by glow-discharge treatment in HH_3, and then, pre-treated antibody molecules (Fab'-SH domain) were covalently immobilized by a bifunctional cross-linking reagent. Using this method, binding sites of the molecule with the supporting material can uniquely be specified. The density of the immobilized antibody molecules was approximately 6 x 10^<11> cm^<-2>, which agreed well with the theoretically estimated one.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 神保泰彦: 医用電子と生体工学. 24. 358 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神保泰彦: 医用電子と生体工学. 24. 62-63 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神保泰彦: 医用電子と生体工学. 25. 155 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Jimbo: Journal of Molecular Electronics.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Jimbo: ""Biosensors and Bioelectronic Systems"Protein Immobilization using Plasma-polymerized Thin Organic Film,"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Jimbo: "Semiconductor Sensor using Plasma-polymerized Organic Film" Japanese Journal of Medical Electronics and Biological Engineering. 24. 358 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Jimbo: "Basic Problems on Semiconductor Sensor for Biological Measurement" Japanese Journal of Medical Electronics and Biological Engineering. 24. 62-63 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Jimbo: "Semiconductor Sensor using Plasma-polymerized Organic Film" Japanese Journal of Medical Electronics and Biological Engineering. 25. 155 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Jimbo: "Orientation-controlled Immobilization of Protein Molecules on Thin Organic Film Deposited by Plasma Technique" Journal of Molecular Electronics.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Jimbo: Protein Immobilization using Plasma-polymerized Thin Organic Film. in Biosensors and Bioelectronic Systems, D.L.Wise Eds., CRC Press,

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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