1988 Fiscal Year Final Research Report Summary
Fundamental studies on the production of copy animal by nuclear transfer in embryos
| Project/Area Number |
61560302
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産学(含草地学)
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| Research Institution | Kyoto University |
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Principal Investigator |
UTSUMI Kyozo Kyoto University, 農学部, 助教授 (90033266)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Masashi Kyoto University, 農学部, 助手 (60093316)
IRITANI Akira Kyoto University, 農学部, 教授 (80026385)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Nuclear transplantation / Reconstracted enbryos / rat / mouse / karyoplast / cytoplst / 核移植胚 / 電気融合 |
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| Research Abstract |
It is generally known that gene expression and its functinal control in early embryos is regulated by a certaun factoplasm. and that in mammalian species only a nucleus of early embryonic cell has a topipotency in proper ooplasm.
This study was desighned to establish the preparation of cytoplasto and karyoplasto from early mouse and rat embryos, the reconstruction of the cytoplsto and karyoplasto by erectrofusion, and the topipotency of those reconstructed embryos. Nucleus and cytoplasm was exchanged to each other in pronuclear embryos. The reconstructed mouse embryos developed to blastocyst stage and the some of the embryos further developed to full term, but those rat embryos no developed beyond 3-cell stage. Nucleocytoplasmic ratio contributed to cell proliferation in embryonic cell. Single blastomere from rat 2-cell embryos was reconstructed to make various nucleocytoplasmic ratio in rat and mouse. When those ratio is less than a half, the developmental ability was detorimentaly decreased. Nuclear and cytoplasm were exchanged between interspecies, mouse and rat pronuclear embryos. No embryos reconstracted developed beyond 3 cell-stage. However, timing of first cleavage to 2-cell stage were regulated under the cytoplasmic controll
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