1988 Fiscal Year Final Research Report Summary
Morphological differentiation of seminiferous epithelium in vitro.
| Project/Area Number |
61570004
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | School of Medicine, Chiba University |
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Principal Investigator |
NAGANO Toshio School of Med. Chiba University, 医学部, 教授 (60009082)
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| Co-Investigator(Kenkyū-buntansha) |
MAEKAWA Mamiko School of Med. Chiba Univ., 医学部, 助手 (20181571)
TOYAMA Yoshiro School of Med. Chiba Univ., 医学部, 講師 (70009637)
TOYOTA Fumie School of Med. Chiba Univ., 医学部, 助教授 (60009751)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Organ culture of seminiferous tubule / Sertoli cell / monoclonal abtibodies to mouse spermatogenic cells / chimera mouse spermatogenesis |
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| Research Abstract |
1) Producing mouse cryptorchid testes for more than 1 month, their seminifous tubule contains Sertoli cells and spermatogonia. The tubule was organ-cultured. The spermatogonia developed into spermatocytes at prophase but no longer developed further. The structure of inter-Sertoli junctions were maintained. 2) Mouse monoclonal antibodies against mouse spermatogenic cells were produced. These antibodies recognized heat-shock proteins, spermatogonia and certain part of the spermatozoon of the mouse. 3) Chimeara aggregations between SL mutant blastocysts were produced. some of the offsprints of male were fertile and baby-mice were obtained from the foster mother mouse. The spermatogenesis of the chimera animal were normal in part and abnormal in the other part. The seminifours epithelium consists of mozaic spermatogenesis, suggesting the sertoli cells originated from the SL were incapable to differentiate the spermatogenic cells but other Sertoli cells were able to differentiate the spermatogenic cells into fertile spermatozoa. 4) Changes of the intramembrane particles and of filipin-sterol complexes were studied in the epididymal spermatozoa of the rat and hamster. The distribution changes during the passage through the epididymis were observed in these spermatozoa.
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