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1988 Fiscal Year Final Research Report Summary

The structure of ribosomal protein genes and the control mechanisms of their expression

Research Project

Project/Area Number 61570117
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionYamagata University

Principal Investigator

TANAKA Tatsuo  Yamagata University School of Medicine, 医学部, 助教授 (70018688)

Co-Investigator(Kenkyū-buntansha) SOH Hidaka  Yamagata University School of Medicine, 医学部, 助手 (00142224)
Project Period (FY) 1986 – 1988
KeywordsRibosomal protein / cDNA cloning / Processed pseudogene / Regenerating liver
Research Abstract

The biogenesis of eucaryotic ribosomes requires an equimolar accumulation of four RNAs and over 70 different ribosomal protein molecules. This stoichiometry is maintained over a wide range of physiological conditions by coordinate regulation. We intended to study the structure of the genes for individual ribosomal proteins to investigate the control mechanisms.
Clones containing cDNA inserts complementary to the mRNAs encoding ribosomal proteins S11, S17, S26, L5, L7a, L27, L30, L31, L35a, and L37a were isolated by hybrid-selected translation from the cDNA library made for 8-9 s poly(A)-RNA from regenerating rat liver. The nucleotide sequences of these cDNA were determined. The structures of these proteins were infered from the nucleotide sequences to confirm the identity of these clones.
Using the recombinat DNAs, we found that the relative abundance of the mRNAs of these proteins increased after partial hepatectomy. Their maximal level was about twice that of normal rat liver, and was achieved 12-18 h after the operation. The abundance decreased near to the normal level after 48 h.
The rat ribosomal protein genes comprise multigene families which contain 15-20 memberes as shown by the Southern blot anaylyses using the cDNAs as probes. We isolated many independent clones of S11,L27,and L35a from a rat genomic library. Analysis of the restiction sites showed that all of them lacked the intervening seguences. Thermal stability of the hybrid molecules beween these genes and the cDNAs indicated that the nucleotide sequences of these genes had various similarities to the the sequences of the cDNAs. These genes lacked the intervening sequence, they contained (A)-rich tracts, and they were flanked by direct repeats. Thus, the ribosomal protein gene families were proven to comprise mostly processed pseudogenes.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Tatsuo Tanaka.: Eur.J.Biochem.154. 523-527 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Tanaka.: Eur.J.Biochem.162. 45-48 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takejiro Kuzumaki.: Biochim.Biophys.Acta. 909. 99-106 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoru Tamura.: Eur.J.Biochem.168. 83-87 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Tanaka.: Eur.J.Biochem.173. 53-56 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Tanaka.: Eur.J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Tanaka: "Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L35a" Eur. J. Biochem.154. 523-527 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsuo Tanaka: "Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L31" Eur. J. Biochem.162. 45-48 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takejiro Kuzumaki: "Rat ribosomal protein L35a multigene family : molecular structure and characterization of three L35a-related" Biochim. Biophys. Acta. 909. 99-106 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru Tamura: "Molecular cloning and nucleotide sequence of cDNA specific for rat ribosomal protein L5" Eur. J. Biochem.168. 83-87 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsuo Tanaka: "Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L27" Eur. J. Biochem.173. 53-56 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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