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1987 Fiscal Year Final Research Report Summary

Is parathyroid hormone present only in secretory granules?

Research Project

Project/Area Number 61570152
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

GOTO Kunihiko  Tohoku Univer. Sch. Med. Univ. Hospital. Senior Instructor, 医学部付属病院, 助手 (70133056)

Project Period (FY) 1986 – 1987
KeywordsPTH / secretory gramele / Imminohistochemistry / protein A-gold / 酵素抗体法 / 金コロイド法
Research Abstract

Electron microscopical immunohistochemistry on human and bovine parathyroid tissue also revealed cytoplasmic activities for parathyroid hormone besides thosein secretory grammles. Immunohistochemical analysis was performed by unlabeledantibody enzyme method indirectly with whole IgG as the first and second antiserum. Anti human PTH-guinea Rg antibody (Toyogyozo) was anti-syntlolic (1-34)PTH. peptide. speeific activity was determined by absorption test and final absonption concentration of antigen(synthetic PTH1-34) against the antibody was 11 g/ml. The postive reaction was generally intensitive in normal parathyroid tissues, less in hyperplasia, and weak in adenoma. However, the normal tissue adjacent to adenoma was less intensitive than adenoma cells.
Electronmicroscopically secretory gramiles were very few but hyghly positive for PTH immunoreactivity and rough ER, and ribosomes were also positive. In order to corroborate the immunohistochemical presence of PTH in cytoplasm, the protein A-gold method was applied for the parathyroid glands of rats which were perfused by glutaraldehyde or periodate-paraformaldehyde in order to prevent the leakage of PTH during useal method of fixation. The positive grams were observed in the cytoplasmic matrix as well as secretory grammles.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 後藤邦彦: 日本内分泌学会雑誌. 62. 403 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤邦彦: 日本病理学会会誌. 75. 492 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Goto: Tohoku Journal of Experimental Medicine.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Goto: American Journal of Pattrology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤邦彦: 細胞.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Goto: Tohoku Journal of Expenmental Medicine.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Goto: "Tissue recenstruction of human and dog anterior pituitany tissue by spimer culture" 3rd International Cell Culture Congress. P-2-14. 106 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Goto: "Is parathyroid hormone present only in secretory gramules?" Tohoku Journal of Experimental Biology. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Goto: "Differentiation gradient reconstructed by spinner culture of human and dog pituitary tissues" American Journal of Pathology. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Goto: "Henidesmosome-like structures of rat Leydig cells are related to cell-cell contact" Tohoku Journal of Experimental Medicine. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Goto: "Cells swell, and generate heat at hunctional expression-rotation model-" Xth International Congress of Pharmacology, Sydney, Australia. P450 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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