1987 Fiscal Year Final Research Report Summary
Studies on Asphyxia: Electron microscopic, immunohistochemical and biochemical studies.
| Project/Area Number |
61570294
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MORITA Masahiko Dept. of Legal Medicine, 医学部, 教授 (00045353)
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| Co-Investigator(Kenkyū-buntansha) |
AKINO Toyoaki Dept. of Biochemistry ( section 1 ), 医学部, 教授 (80045377)
TABATA Noriko Dept. of Legal Medicine, 医学部, 助手 (30128524)
森田 匡彦 札幌医科大学, 医学部, 教授 (00045353)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Asphyxia / Hypoxia / Lung surfactant / Immunohistochemistry / モノクローナル抗体 |
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| Research Abstract |
Studies on asphyxia were performed by using rats inhaled air with decreased oxygen. The air was prepared by using fluorometer, mixing carbon dioxide, methane and carbon monoxide gases. The electron microscopic study showed the debris-like structures in which the lattice like structure and/or tubular myeline which is well-known as the morphological expression of lung surfactant. Biological analysis revealed that the protein and phospholipids increased as 5 and 2 and/or 3 times as much compared with those of normal rat's lung lavage. Immunohistochemical research was also performed using the monoclonal anti-body to the human lung surfactant apo-protein. The active secretion of the surfactant in such hypoxic state mentioned above and in the human lung in a case of strangulation as well by this study. In conclusion, the increase in the secretion of the lung surfactant in hypoxic state, should be attributed to the decrease of the oxygen in the inhaled air and/or decreased tension of oxygen in the alveoli.
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