1987 Fiscal Year Final Research Report Summary
Research for retroviruses in thymus cells of myasthenia gravis and their involvement in the pathogeneses of myasthenia gravis
| Project/Area Number |
61570394
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | The Kitassto Institute |
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Principal Investigator |
ONO Kairo Departmrnt of Virology,The Kitasato Institute, ウイルス部, 副部長 (80160901)
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| Co-Investigator(Kenkyū-buntansha) |
HIBI Toshifumi Department of Internal Medicine,The Kitasato Institute Hospital, 病院内科, 医長 (50129623)
MORITA Minoru Department of Virology,The Kitasato Institute, ウイルス部, 技師補
KOMATSU Haruko Department of Virology,The Kitasato Institute, ウイルス部, 技師 (20106678)
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| Project Period (FY) |
1986 – 1987
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| Keywords | myasthenia graves / thymus / retrovirus / cDNA / ウイルス遺伝子のクローニング |
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| Research Abstract |
Retrovirus-like particles were demonstrated in cultured thymus cells(thymomas and thymus hyperplasias)of parients with myasthenia gravis.Human T cells were infected with these particles.Reverse transcriptase activity was eluted in the culture fluids of these T cells.Virus particles were also detected in these T cells.In Southern blot,cDNA prepared by endogenous polymerase reaction with detergent-treated virons from virus-infected T cells hybridized to DNAs of virus-infected T cells.Antibodies to such virus particles were detected in sera of parients with myasthenia gravis.Virus particles were also demonstrated in sv 40 DNA (ori^-)-transformed human thymus epithelial cell lines.
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