1987 Fiscal Year Final Research Report Summary
The Production of Congenital Melformations by Presensitization of Tissue Antisera
| Project/Area Number |
61570463
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
MIYATA Koichiro Faculty of Medicine, Kagoshima University, 医学部, 教授 (30041411)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Eiji Faculty of Medicine, Kagoshima University, 医学部, 助手 (40174776)
KAWAKAMI Kiyoshi Faculty of Medicine, Kagoshima University, 医学部附属病院, 講師 (50152921)
YOSHINAGA Masao Faculty of Medicine, Kagoshima University, 医学部附属病院, 講師 (10145469)
ONO Seigo Faculty of Medicine, Kagoshima University, 医学部, 助教授 (00094128)
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| Project Period (FY) |
1986 – 1987
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| Keywords | experimental teratology / antiheart serum / antikidny serum / cardiovascular / malformations / yolk sac dysfunction / presensitization |
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| Research Abstract |
In order to produce malformations in rat embryos, the following experiments were undertaken using tissue antisera. AHS+AHS group; female rats(5 weeks old) were sensitized with rabbit anti-rat-heart-serum(AHS), and then injected with 9 ml/kg of AHS on day 9 of gestation. AHS+NRS group; presensitized with AHS, and injected with 9 ml/kg of normal rabbit serum(NRS). NRS+AHS group; presensitized with NRS, and injected with AHS. NRS+NRS group; presensitized with NRS, and injected with NRS. AKS+AHS group;presensitized with small doses of anti-kidney serum(AKS) and injected with AHS. AHS+AKS group; presensitized with AHS, and injected with a small dose of AKS.
The results were as follows:
1) The AHS+AHS, AKS+AHS, and AHS+AKS groups had significantly higher incidence of resorption and malformations than the three control groups.
2) The main malformations were microphthalmia, ventricular septal defect, hydrocephaly, pulmonary stenosis, general edema, and aortic arch anomalies.
3) No antinuclear antibody was detected in the maternal sera by the fluorescent antibody technique.
4) No complete heart block was found in the fetuses by electrocardiography.
5) The fluorescent antibody technique demonstrated localization of rat IgG and rat C_3 on the maternal myocardium, but a light-microscopic study revealed no myocarditis or endocarditis in either the maternal or fetal heart.
These results suggest that both the existence of the immune complexes of antibodies against AHS or AKS and the administration of AHS or AKS on day 9 are necessary to cause yolk sac dysfunction or other teratogenic changes.
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