1987 Fiscal Year Final Research Report Summary
Production of human monoclonal antibodies using lymphocytes from cancer patients
| Project/Area Number |
61570498
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kitasato University |
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Principal Investigator |
ISHIYAMA Shigeo Kitasato University School of Medicine, Professor, 医学部, 教授 (20050354)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Kotaro Kitasato University School of Medicine, assstant, 医学部, 助手 (40155338)
ETO Thikaru Kitasato University School of Medicine, instractor, 医学部, 講師 (20137920)
TAKEZAKI Shinichiro Kitasata University School of Medicine, instractor (40129250)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Monoclonal antibody / Human-human hybridoma / Auto-antibody / 自己抗体 |
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| Research Abstract |
In the present project, we intended to profuce human monoclonal antibody using lymphocytes from cancer or pemphigus/pemphigoid patients. Although we failed to produce tumor-specific monoclonal antibody, we have produced several interesting human monoclonal antibodies using human-human hybridoma technique. These antibodies were produced by fusing lymphocytes from peripheral blood or lymph node with human myeloma cell line LICR-LON-HMy_2. One antibody, F32.7,recognized cell surface of upper epidermal keratinocytes. Another antibody, F28.5, reacted with whole epidermis, appendageal cells and most of mesenchymal cells in the dermis in a cytoplasmic pattern suggesting that it reacted with keratin(s) and some other intermediate filaments including vimentin. Other antibodies reacted specifically with cell surface of upper epidermal layers, hair follicles or sabaceous glands. These antibodies would be useful in studying the rolls of auto-antibodies in skin diseases.
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