1988 Fiscal Year Final Research Report Summary
Immunohistochemical study on the post-mortem brain of schizophrenics.
| Project/Area Number |
61570523
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SATOH Keiji Shiga University of Medical Science, Lecturer, 医学部, 講師 (80093417)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Schizophrenia / Immunohistochemistry / Neurotransmitter / Peptide / Post-mortem Brain / 霊長類 |
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| Research Abstract |
In our laboratory, immunohistochemical studies of the cerebral cortex and limbic structures are presently performed on post-portem brain materials from control subjects and schizophrenic patients. primary antibodies raised against tyrosine hydroxylase, serotonin-bSA conjugate, substance P, somatostatin and neurotensin were used to investigate the central monoaminergic and peptiderbic systems in the forebrain. Avidin-liotin-peroxidase method of Hsu was applied to visualize the immunoreactionend product. A preliminary study on monkey brains revealed a marked variation between peptidergic systems in the distribution of neuronal cell bodies and their processes. The highest density of fibers, immunoreactive to substance P, somatostatin and neurotensin, was observed in the anterior cingulate cortex (area 24 of Walker). The same area also showed the highest density of distribution of fibers immunoreactive to tyrosine hydroxylase in the cerebral cortex. A high correlation was seen particularly in the superficial layers of monkey cortex. A similar pattern of distribution of substance P-containing fibers was demonstrated in the frontal cortex of control human materials. We are now applying the same technique on post-mortem brains of chronic schizophrenics. This technique has been shown to be reliable in many neuropatholigical studies, and to be essential in the advencement of clinical neuroscience research.
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Research Products
(20 results)
