1988 Fiscal Year Final Research Report Summary
Analysis of pathophysiological states and detection of AIDS retrovirus in hemophiliacs
| Project/Area Number |
61570585
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TOSHIO HATTORI Second Department of Internal Medicine, Kummamoto University Medical School, 医学部, 講師 (30172935)
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| Project Period (FY) |
1986 – 1988
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| Keywords | AIDS / HIV / monoclonal antibody / VAK4 |
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| Research Abstract |
Acquired immune deficiency syndrome (AIDS) is etiologically associated with human immuno deficiency virus type 1 (HIV-1). We have surveyed anti-HIV-1 antibody in Japanese hemophiliacs by Western blotting (W-B) method. Over 50% of hemophiliacs were positive for anti-HIV-1 antibody. In order to detect HIV-1 infected cells in infected individuals, we have raised monoclonal antibodies (VAK3,4,5) against structures of HIV. These antibodies specifically react with a major core protein (p24) of HIV-1. Commpetitive binding study showed that these monoclonal antibodies react with the same epitope of p24. VAK4 monoclonal antibody reacts with all the strains of HIV-1, HIV-2 as well as SIV, suggesting that VAK4 reacts with a conserved epitope of HIV-related viruses. Proteins from Esherichia Coli containing plasmids of a whole region of p24 and a part of p15 were positive for VAK4, while those from Esherichia Coli containing the plasmid, which lacked 100 amino acids of carboxyl terminus of p24 reacted with VAK4. These findings suggest that there were conserved sequences in carboxyl terminus of p24. Further delineation of the epitope is now in progress and would be helpful to develop a sensitive assay system for detection of HIV-1 antigen in the sera of HIV-1 and related viruses infected individuals.
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