1987 Fiscal Year Final Research Report Summary
Clinical study of monoclonal antibodies againt pancreas cancer associated antigens
| Project/Area Number |
61570650
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University Medical School |
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Principal Investigator |
SHIMANO Takashi Osaka University Medical School, 医学部, 助手 (80144476)
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| Co-Investigator(Kenkyū-buntansha) |
MONDEN Takushi Osaka University Medical School, 医学部, 助手 (20174477)
KOBAYASHI Tetsuro Osaka University Medical School, 医学部, 助手 (40162002)
INAJI Hedio Osaka University Medical School, 医学部, 助手 (50159943)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Immunodiagnosis / Monoclonal antibodies / Pancreas cancer-associated antigen / 酵素免疫測定法 |
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| Research Abstract |
Monoclonal antibodies against PCAA_p(pancreas cancer-associated antigen from ascites fluids of a pancreatic cancer patient) were produced. The two monoclonal antibodies were selected. The two MoAbs reacted with cancer cells and intraductal mucin-like products in well differentiated pancreatic cancer tissues. PCAA have usually expressed in normal colonic mucosa (PCAA_c), however, those two MoAbs reacted scarecely with normal colonic mucosa. A sand wich enzyme immunoassay was newly developed to measure circulating PCAA_p. From a group of 32 healthy volunteers, an upper limit of 2.06 micro g/ml has been tentatively determined. An elevated PCAA_p was shown in 72%(23/32) of patients with pancreatic cancer,65%(13/20) of patients with bile duct cancer, 60%(21/35) of patients with hepatoma and 10%(6/60) of patients with benign hepatobilialy disease. No correlations were observed between serum CA19-9 and PCAA_p.
Monoclonal antibodies against fetal pancreas (FP-1,FP-2 and FP-3) were characterized. FP-1,FP-2 and FP-3 reacted specifically with ductal cells, acinar cells and islet cells, respectively. FP-1 recongnized peptide and reacted with 15/21 of pancreatic cancer tissues. FP-2 and FP-3 recongnized carbohydrate and FP-3 reacted with 8/13 of APUDOMAS (medullary thyroid carcinomas,carcinoids and pheochromocytomas).
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