1988 Fiscal Year Final Research Report Summary
Behavioral and biochemical study of brain edema
Project/Area Number |
61570701
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Cerebral neurosurgery
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Research Institution | Kobe University |
Principal Investigator |
YAMAGUCHI Michio School of Allied Medical Sciences, Kobe University, Proffessor, 医療技術短期大学部, 教授 (40030861)
|
Project Period (FY) |
1986 – 1988
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Keywords | Experimental brain edema / Water intoxication / Active avoidance / Rat / Lysosome / 蛋白分解酵素阻害剤 / ステロイド / AVS |
Research Abstract |
Behavioral study was performed on rats with experimental brain edema. Water intoxication brain edema was firstly produced by the injection of distilled water intraperitoneally. The volumes of water for injection were 5, 10, or 20 % of the body weight. No vasopression was administered because of its pharmacological side action to the central nervous system. Two hours after the water loading, the brain was quickly taken by decapitation and contents of water, sodium, and potassium were determined. Increase of water content was significant in the brain tissue when water was given by the volume of 10 % or more of body weight. The contents of sodium and potassium were evidently reduced to some extent in the edematous tissue. These animals were tested for their ability to learn the active avoidance from electric shock. The water loading of 10 % body weight or over always gave the disturbed learning of the active avoidance. Another model of experimental brain edema was produced the intracerebral implantation of small amounts of silver nitrate, as a inflammation-causing agent. A localized brain edema with blood-brain barrier dysfunction could be produced by this method. Twenty four hours later, the brain specimens were analysed on the contents of water, sodium, and potassium. This method was useful for the formation of the experimental brain edema, too. Water and sodium contents were significantly increased in the edematous tissue. The activity of lysosome enzyme, beta-Glucuronidase, were also determined. This enzyme activity was increased in the edematous tissue. This increase was successfully reduced by the intraperitoneal administration of protease inhibitors, Urinastatin or Aprotinin. Antioxidant (AVS) and steroid (Betamethasone) were also effective to this brain edema model. AVS was also effective to prevent the increase of lipoperoxide in the edematous brain tissue. No behavioral test could be carried out at this time.
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Research Products
(6 results)