1988 Fiscal Year Final Research Report Summary
Experimental study on secondary living bone graft by vascular implantation
| Project/Area Number |
61570725
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
MASUHARA Kenji Dean. Dept. of Orthopaedic Surgery, 医学部, 学長 (90075086)
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| Co-Investigator(Kenkyū-buntansha) |
YAJIMA Hiroshi Clinical Assistant. Dept. of Orthopaedic Surgery, 医学部, 助手
TAMAI Susumu Professor. Dept. of Orthopaedic Surgery, 医学部, 教授 (10075088)
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| Project Period (FY) |
1986 – 1988
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| Keywords | vascular bundle implantation / vascularized bone graft / 骨移植 / 血管柄付骨移植 |
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| Research Abstract |
The purpose of this study is to demonstrate the possibility of creating a new nutrient vascular system of the tibia with a vascular bundle. implantation and applying it as a secondary living bone graft.
Using Fisher strain F-344 adult rats, the right saphenous artery and vein were elevated as a vascular bundle, and this was implanted to the intramedullary cavity of an intact tibia (Group A), and on the periosteum of an intact tibia (Group B), and to the intramedullary cavity of an isolated tibia shaft (Group C). At several intervals after the poeration, the island vascularized tibia, nourished by the implanted saphenous vascular bundle, was elevated on the vascular pedicle and transferred to the abdominal subcutaneous space. Thereafter, microangiographic, histological, and fluorcscent labeling studies were performed.
The follwing results were obtained: (1) In Group A, survival of the bone cells was noted histologically, and on the undercalcified specimens with fluorescent labeling, the bone cells were found to be viable after the transfer to the abdominal subcutaneous space. (2) In Group B, only the bone cells in the outer layer of a cortex were revitalized after the transfer. (3) In Group C, the vascular proliferation from the implanted vessels was seen on the microangiogram. And this proliferation was more conspicuous than that of Group A. Histologically, endosteal and periosteal osteogenesis were noted. The fluorochrome uptakes were seen in some Haversian canals, but a majority of the cortex was still necrotic even at five weeks after the implantation of a vascular bundle.
The results suggest that "SECONDARY LIVING BONE GRAFT" by the implantation of a vascular bundle to the intramedullary cavity of an intact bone is realized, and can be applied clinically as a new source of vascularized bone grafts.
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