1987 Fiscal Year Final Research Report Summary
^<31>P-NMR studies of the Activation of the Mammalian Eggs
| Project/Area Number |
61570778
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
MUTSUO Ishikawa Asahikawa Medical College, Assoicate Professor, 医学部, 助教授 (20002131)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEO Shibata Asahikawa Medical College, Staff associate, 医学部, 助手 (70154242)
MITSUNORI Kasamo Asahikawa Medical College, Staff associate, 医学部, 助手 (90177361)
KUNIO Tanaka Asahikawa Medical College, Associate Professor, 助教授 (20041840)
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| Project Period (FY) |
1986 – 1987
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| Keywords | intracellular pH / cryopreservation / ^<31>P-NMR / 凍結融解法 |
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| Research Abstract |
^<31>P-NMR signals assigned to intracellular adenosine nucleotides and to inorganic phosphate were studied in the mammalian eggs around fertilization. Above mentioned purpose, the method of cryopreservation and dilution of the fertiilized and fertilized eggs of ICR mouse have been established. The intracellular oxygen tention and pH changes in hamster embryos at various stages of development was examined using a microelectrode technique. Slight but significant increases in oxygen tenton in embryos occurred at the 2 cell stage and each successive stage. And also, slight increase of intracellular pH in embryos occurred at 2 cell stage. ^<31>P-NMR spectra of perfused rabbit ovaries abtained around ovulation.
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